![](/images/graphics-bg.png)
Expression of Angiotensin II Receptor-1 in Human Articular Chondrocytes
Joint Authors
Beppu, Moroe
Nakamura, Hiroshi
Shimizu, Hiroyuki
Masuko, Kayo
Inaba, Yutaka
Yudoh, Kazuo
Kato, Tomohiro
Murata, Minako
Matsuo, Kosuke
Kawakami, Yuki
Saito, Tomoyuki
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Besides its involvement in the cardiovascular system, the renin-angiotensin-aldosterone (RAS) system has also been suggested to play an important role in inflammation.
To explore the role of this system in cartilage damage in arthritis, we investigated the expression of angiotensin II receptors in chondrocytes.
Methods.
Articular cartilage was obtained from patients with osteoarthritis, rheumatoid arthritis, and traumatic fractures who were undergoing arthroplasty.
Chondrocytes were isolated and cultured in vitro with or without interleukin (IL-1).
The expression of angiotensin II receptor types 1 (AT1R) and 2 (AT2R) mRNA by the chondrocytes was analyzed using reverse transcription-polymerase chain reaction (RT-PCR).
AT1R expression in cartilage tissue was analyzed using immunohistochemistry.
The effect of IL-1 on AT1R/AT2R expression in the chondrocytes was analyzed by quantitative PCR and flow cytometry.
Results.
Chondrocytes from all patient types expressed AT1R/AT2R mRNA, though considerable variation was found between samples.
Immunohistochemical analysis confirmed AT1R expression at the protein level.
Stimulation with IL-1 enhanced the expression of AT1R/AT2R mRNA in OA and RA chondrocytes.
Conclusions.
Human articular chondrocytes, at least partially, express angiotensin II receptors, and IL-1 stimulation induced AT1R/AT2R mRNA expression significantly.
American Psychological Association (APA)
Kawakami, Yuki& Matsuo, Kosuke& Murata, Minako& Yudoh, Kazuo& Nakamura, Hiroshi& Shimizu, Hiroyuki…[et al.]. 2012. Expression of Angiotensin II Receptor-1 in Human Articular Chondrocytes. Arthritis،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-488045
Modern Language Association (MLA)
Kawakami, Yuki…[et al.]. Expression of Angiotensin II Receptor-1 in Human Articular Chondrocytes. Arthritis No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-488045
American Medical Association (AMA)
Kawakami, Yuki& Matsuo, Kosuke& Murata, Minako& Yudoh, Kazuo& Nakamura, Hiroshi& Shimizu, Hiroyuki…[et al.]. Expression of Angiotensin II Receptor-1 in Human Articular Chondrocytes. Arthritis. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-488045
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488045