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The Therapeutic Effect of Cytokine-Induced Killer Cells on Pancreatic Cancer Enhanced by Dendritic Cells Pulsed with K-Ras Mutant Peptide
Joint Authors
Zhang, Xin
Feng, Hongbo
Wang, Zhongyu
Luo, Haifeng
Tan, Guang
Source
Clinical and Developmental Immunology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
This study is to investigate the role of the CIKs cocultured with K-ras-DCs in killing of pancreatic cancer cell lines, PANC-1 (K-ras+) and SW1990 (K-ras−).
Methods.
CIKs induced by IFN-γ, IL-2, and anti-CD3 monoantibody, K-ras-DCCIKs obtained by cocultivation of k-ras-DCs and CIKs.
Surface markers examined by FACS.
IFN-γ IL-12 ,CCL19 and CCL22 detected by ELISA.
Proliferation of various CIKs tested via 3H-TdR.
Killing activities of k-ras-DCCIKs and CTLs examined with 125IUdR.
Results.
CD3+CD56+ and CD3+CD8+ were highly expressed by K-ras-DCCIKs.
In its supernatant, IFN-γ, IL-12, CCL19 and CCL22 were significantly higher than those in DCCIK and CIK.
The killing rate of K-ras-DCCIK was greater than those of CIK and CTL.
CTL induced by K-ras-DCs only inhibited the PANC-1 cells.
Conclusions.
The k-ras-DC can enhance CIK's proliferation and increase the killing effect on pancreatic cancer cell.
The CTLs induced by K-ras-DC can only inhibit PANC-1 cells.
In this study, K-ras-DCCIKs also show the specific inhibition to PANC-1 cells, their tumor suppression is almost same with the CTLs, their total tumor inhibitory efficiency is higher than that of the CTLs.
American Psychological Association (APA)
Tan, Guang& Zhang, Xin& Feng, Hongbo& Luo, Haifeng& Wang, Zhongyu. 2011. The Therapeutic Effect of Cytokine-Induced Killer Cells on Pancreatic Cancer Enhanced by Dendritic Cells Pulsed with K-Ras Mutant Peptide. Clinical and Developmental Immunology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-488123
Modern Language Association (MLA)
Tan, Guang…[et al.]. The Therapeutic Effect of Cytokine-Induced Killer Cells on Pancreatic Cancer Enhanced by Dendritic Cells Pulsed with K-Ras Mutant Peptide. Clinical and Developmental Immunology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-488123
American Medical Association (AMA)
Tan, Guang& Zhang, Xin& Feng, Hongbo& Luo, Haifeng& Wang, Zhongyu. The Therapeutic Effect of Cytokine-Induced Killer Cells on Pancreatic Cancer Enhanced by Dendritic Cells Pulsed with K-Ras Mutant Peptide. Clinical and Developmental Immunology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-488123
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488123