Chromatographic Removal of Endotoxins : A Bioprocess Engineer's Perspective
Joint Authors
Chew, Jia Han
Danquah, Michael K.
Ongkudon, Clarence M.
Liu, Boyin
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Gram-negative bacteria are widely used for the production of gene-based products such as DNA vaccines and bio-drugs, where endotoxin contamination can occur at any point within the process and its removal is of great concern.
In this article, we review the structures of endotoxin and the effects that it causes in vivo.
The endotoxin removal strategies are also discussed in the light of the different interaction mechanisms involved between endotoxins and bioproducts particularly plasmid DNA and proteins.
For most cases, endotoxin removal is favoured at a highly ionic or acidic condition.
Various removal methods particularly chromatography-based techniques are covered in this article according to the relevant applications.
American Psychological Association (APA)
Ongkudon, Clarence M.& Chew, Jia Han& Liu, Boyin& Danquah, Michael K.. 2012. Chromatographic Removal of Endotoxins : A Bioprocess Engineer's Perspective. ISRN Chromatography،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-488173
Modern Language Association (MLA)
Ongkudon, Clarence M.…[et al.]. Chromatographic Removal of Endotoxins : A Bioprocess Engineer's Perspective. ISRN Chromatography No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-488173
American Medical Association (AMA)
Ongkudon, Clarence M.& Chew, Jia Han& Liu, Boyin& Danquah, Michael K.. Chromatographic Removal of Endotoxins : A Bioprocess Engineer's Perspective. ISRN Chromatography. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-488173
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488173