![](/images/graphics-bg.png)
Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats
Joint Authors
Liou, Dann-Ying
Huang, Ming-Chao
Yu, Nien-Chu
Tsai, May-Jywan
Cheng, Henrich
Weng, Ching-Feng
Shyue, Song-Kun
Tam, Kabik
Huang, Wen-Cheng
Kuo, Fu-San
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Prostacyclin (PGI2), a potent vasodilator and platelet antiaggregatory eicosanoid, is cytoprotective in cerebral circulation.
It is synthesized from arachidonic acid (AA) by the sequential action of cyclooxygenase- (COX-) 1 or 2 and prostacyclin synthase (PGIS).
Because prostacyclin is unstable in vivo, PGI2 analogs have been developed and demonstrated to protect against brain ischemia.
This work attempts to selectively augment PGI2 synthesis in mixed glial culture or in a model of Parkinson’s disease (PD) by direct adenoviral gene transfer of prostacyclin biosynthetic enzymes and examines whether it confers protection in cultures or in vivo.
Confluent mixed glial cultures actively metabolized exogenous AA into PGE2 and PGD2.
These PGs were largely NS398 sensitive and considered as COX-2 products.
Gene transfer of AdPGIS to the cultures effectively shunted the AA catabolism to prostacyclin synthesis and concurrently reduced cell proliferation.
Furthermore, PGIS overexpression significantly reduced LPS stimulation in cultures.
In vivo, adenoviral gene transfer of bicistronic COX-1/PGIS to substantia nigra protected 6-OHDA- induced dopamine depletion and ameliorated behavioral deficits.
Taken together, this study shows that enhanced prostacyclin synthesis reduced glial activation and ameliorated motor dysfunction in hemiparkinsonian rats.
Prostacyclin may have a neuroprotective role in modulating the inflammatory response in degenerating nigra-striatal pathway.
American Psychological Association (APA)
Tsai, May-Jywan& Weng, Ching-Feng& Yu, Nien-Chu& Liou, Dann-Ying& Kuo, Fu-San& Huang, Ming-Chao…[et al.]. 2013. Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-488180
Modern Language Association (MLA)
Tsai, May-Jywan…[et al.]. Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-488180
American Medical Association (AMA)
Tsai, May-Jywan& Weng, Ching-Feng& Yu, Nien-Chu& Liou, Dann-Ying& Kuo, Fu-San& Huang, Ming-Chao…[et al.]. Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-488180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488180