Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms
Joint Authors
El Khayat, Engy Hamdy
Ali, Hala Abdel Salam
Omar, Nancy Younis
Harfoush, Reem Abdel Hameed
Source
International Journal of Microbiology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals.
This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A (spa) and coagulase (coa) gene polymorphisms to verify their ability in assessing the relatedness of isolates.
Seventy-five MRSA isolates, from different ICUs of Alexandria University Main Hospital, were characterized using antibiotyping and PCR-RFLP analysis of coa and spa genes.
Thirty-two antibiotypes were identified.
coa gene PCR generated 3 types and 10 subtypes of band patterns.
HaeIII restriction digestion of amplified coa gene products produced 5 major banding patterns and 12 subtypes.
spa gene PCR products generated 4 major and 11 minor types, and their HaeII restriction digestion showed 5 major and 12 minor banding patterns.
The combined coa and spa RFLP patterns generated 22 combined R types.
Typing using coa PCR and PCR-RFLP had the same discriminatory index (DI) value (0.64), which was comparable to that of both spa PCR and PCR-RFLP techniques (0.68).
The combined grouping increased the DI value to 0.836.
The current study revealed that testing for multiple gene polymorphisms is more useful for local epidemiologic purposes.
American Psychological Association (APA)
Omar, Nancy Younis& Ali, Hala Abdel Salam& Harfoush, Reem Abdel Hameed& El Khayat, Engy Hamdy. 2014. Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms. International Journal of Microbiology،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-488212
Modern Language Association (MLA)
Omar, Nancy Younis…[et al.]. Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms. International Journal of Microbiology No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-488212
American Medical Association (AMA)
Omar, Nancy Younis& Ali, Hala Abdel Salam& Harfoush, Reem Abdel Hameed& El Khayat, Engy Hamdy. Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms. International Journal of Microbiology. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-488212
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488212