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Isolation of Cellulolytic Bacillus subtilis Strains from Agricultural Environments
Joint Authors
Lee, Shin-Chan
Cho, Young-Yun
Oh, Hyun-Jeong
Ko, Young Hwan
Kim, Yu-Kyoung
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The bioconversion of cellulose and hemicellulose to soluble sugars is important for global stabilization and a sustainable human society.
Here, hundreds of cellulolytic bacteria were screened and isolated from soil, compost, and animal waste slurry in Jeju Island, South Korea.
Among the isolates, three strains, SL9-9, C5-16, and S52-2, showing higher potential for practical uses were purified on carboxymethyl cellulose (CMC) agar plates and identified as Bacillus subtilis strains by morphological, physiological, and biochemical characterization and 16S rRNA gene analysis.
The production patterns of cellulose or hemicellulose-degrading enzymes were investigated during cell culture.
All three isolated strains produced CMCase, Avicelase, β-glucosidase, and xylanase enzymes, which suggested synergic cellulolytic systems in Bacillus subtilis.
The enzymes showing CMCase, Avicelase, and xylanase activities existed in cell-free culture supernatant, meanwhile β-glucosidase activity was detected in cell debris suggesting that three of the enzymes, including CMCase, Avicelase, and xylanase, were extracellular, and β-glucosidase was cell membrane bound.
The three isolates, SL9-9, C5-16, and S52-2, were not the same strains, presenting slight differences in biochemical characteristics, 16S rRNA gene sequences, and cellulolytic enzyme activities.
American Psychological Association (APA)
Kim, Yu-Kyoung& Lee, Shin-Chan& Cho, Young-Yun& Oh, Hyun-Jeong& Ko, Young Hwan. 2012. Isolation of Cellulolytic Bacillus subtilis Strains from Agricultural Environments. ISRN Microbiology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-488232
Modern Language Association (MLA)
Kim, Yu-Kyoung…[et al.]. Isolation of Cellulolytic Bacillus subtilis Strains from Agricultural Environments. ISRN Microbiology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-488232
American Medical Association (AMA)
Kim, Yu-Kyoung& Lee, Shin-Chan& Cho, Young-Yun& Oh, Hyun-Jeong& Ko, Young Hwan. Isolation of Cellulolytic Bacillus subtilis Strains from Agricultural Environments. ISRN Microbiology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-488232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488232