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Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy
Joint Authors
Palets, Dmytro B.
Lyubchenko, Yuri L.
Filenko, Nina A.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly.
Internucleosomal interaction is required for the formation of higher-order chromatin structures.
Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well.
To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging.
The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template.
Di-nucleosomes in close proximity were observed, but their population was low.
The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes.
The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed.
American Psychological Association (APA)
Filenko, Nina A.& Palets, Dmytro B.& Lyubchenko, Yuri L.. 2011. Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy. Journal of Amino Acids،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-488254
Modern Language Association (MLA)
Filenko, Nina A.…[et al.]. Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy. Journal of Amino Acids No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-488254
American Medical Association (AMA)
Filenko, Nina A.& Palets, Dmytro B.& Lyubchenko, Yuri L.. Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy. Journal of Amino Acids. 2011. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-488254
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488254