Identification and Validation of Expressed Sequence Tags from Pigeonpea (Cajanus cajan L.) Root
Joint Authors
Bhandare, Prithviraj P.
Joshi, Shourabh
Sonkawade, Swati
Kulkarni, Pramod B.
Yadav, Shailesh
Patil, Vinod Kumar
Kumar, Ravi Ranjan
Naik, G. R.
Source
International Journal of Plant Genomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Pigeonpea (Cajanus cajan (L) Millsp.) is an important food legume crop of rain fed agriculture in the arid and semiarid tropics of the world.
It has deep and extensive root system which serves a number of important physiological and metabolic functions in plant development and growth.
In order to identify genes associated with pigeonpea root, ESTs were generated from the root tissues of pigeonpea (GRG-295 genotype) by normalized cDNA library.
A total of 105 high quality ESTs were generated by sequencing of 250 random clones which resulted in 72 unigenes comprising 25 contigs and 47 singlets.
The ESTs were assigned to 9 functional categories on the basis of their putative function.
In order to validate the possible expression of transcripts, four genes, namely, S-adenosylmethionine synthetase, phosphoglycerate kinase, serine carboxypeptidase, and methionine aminopeptidase, were further analyzed by reverse transcriptase PCR.
The possible role of the identified transcripts and their functions associated with root will also be a valuable resource for the functional genomics study in legume crop.
American Psychological Association (APA)
Kumar, Ravi Ranjan& Yadav, Shailesh& Joshi, Shourabh& Bhandare, Prithviraj P.& Patil, Vinod Kumar& Kulkarni, Pramod B.…[et al.]. 2014. Identification and Validation of Expressed Sequence Tags from Pigeonpea (Cajanus cajan L.) Root. International Journal of Plant Genomics،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-488373
Modern Language Association (MLA)
Kumar, Ravi Ranjan…[et al.]. Identification and Validation of Expressed Sequence Tags from Pigeonpea (Cajanus cajan L.) Root. International Journal of Plant Genomics No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-488373
American Medical Association (AMA)
Kumar, Ravi Ranjan& Yadav, Shailesh& Joshi, Shourabh& Bhandare, Prithviraj P.& Patil, Vinod Kumar& Kulkarni, Pramod B.…[et al.]. Identification and Validation of Expressed Sequence Tags from Pigeonpea (Cajanus cajan L.) Root. International Journal of Plant Genomics. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-488373
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488373