Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein
Joint Authors
Chandran, Dev
Nimmagadda, Sridevi V.
Villuppanoor, Srinivasan Alwar
Lingala, Rajendra
Aavula, Shukra M.
Sula, Samuel
Biradhar, Neelakantam
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Information Technology and Computer Science
Abstract EN
Recombinant antibody phage display technology is a vital tool that facilitates identification of specific binding molecules to a target enabling the rapid generation and selection of high affinity, fully human, or mouse antibody product candidates essentially directed towards disease target appropriate for antibody therapy.
In this study, a recombinant single-chain Fv antibody fragment (scFv) A11 was isolated from immune spleen cells obtained from mice immunized with inactivated rabies virus (Pasteur strain) using standard methodology and was characterized for its specificity towards the rabies virus glycoprotein.
Epitope mapping using peptide libraries and truncated glycoprotein polypeptides suggested that A11 bound to the antigenic site II of rabies glycoprotein against which a majority of rabies virus neutralizing antibodies are directed.
The use of the above technology could, therefore, allow development of scFvs with different specificities against the rabies glycoprotein as an alternative to the more cumbersome protocols used for the development of monoclonal antibodies.
American Psychological Association (APA)
Aavula, Shukra M.& Nimmagadda, Sridevi V.& Biradhar, Neelakantam& Sula, Samuel& Chandran, Dev& Lingala, Rajendra…[et al.]. 2011. Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-488406
Modern Language Association (MLA)
Aavula, Shukra M.…[et al.]. Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein. Biotechnology Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-488406
American Medical Association (AMA)
Aavula, Shukra M.& Nimmagadda, Sridevi V.& Biradhar, Neelakantam& Sula, Samuel& Chandran, Dev& Lingala, Rajendra…[et al.]. Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-488406
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488406