Cloning of Acyl-ACP Thioesterase FatA from Arachis hypogaea L. and Its Expression in Escherichia coli
Joint Authors
Tang, Gui-ying
Chen, Gao
Zhang, Yan
Li, Lan
Bi, Yu-ping
He, Qing-fang
Peng, Zhen-ying
Xuan, Ning
Shan, Lei
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L.
by 3′-RACE.
Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and has 50–70% identity with FatA from other plants.
Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A.
hypogaea L., but most strongly in the immature seeds harvested at 60 days after pegging.
Heterologous expression of AhFatA in Escherichia coli affected bacterial growth and changed the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid.
These results indicate that AhFatA is at least partially responsible for determining the high palmitoleic acid and oleic acid composition of E.
coli.
American Psychological Association (APA)
Chen, Gao& Peng, Zhen-ying& Shan, Lei& Xuan, Ning& Tang, Gui-ying& Zhang, Yan…[et al.]. 2012. Cloning of Acyl-ACP Thioesterase FatA from Arachis hypogaea L. and Its Expression in Escherichia coli. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-488446
Modern Language Association (MLA)
Chen, Gao…[et al.]. Cloning of Acyl-ACP Thioesterase FatA from Arachis hypogaea L. and Its Expression in Escherichia coli. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-488446
American Medical Association (AMA)
Chen, Gao& Peng, Zhen-ying& Shan, Lei& Xuan, Ning& Tang, Gui-ying& Zhang, Yan…[et al.]. Cloning of Acyl-ACP Thioesterase FatA from Arachis hypogaea L. and Its Expression in Escherichia coli. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-488446
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488446