Could Upregulated Hsp70 Protein Compensate for the Hsp90-Silence-Induced Cell Death in Glioma Cells?
Joint Authors
Khan, Zarine
Shervington, Amal
Munje, Chinmay
Shervington, Leroy
Source
International Journal of Brain Science
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The molecular chaperone heat shock protein 90 alpha (Hsp90α) has been recognized in various tumours including glioma.
This pilot study using a proteomic approach analyses the downstream effects of Hsp90 inhibition using 17-allylamino-17-demethoxygeldanamycin (17AAG) and a short hairpin RNA (shRNA) oligonucleotide targeting hsp90α (shhsp90α) in the U87-MG glioma cell line.
Preliminary data coupled with bioinformatic analysis identified several known and unknown Hsp90 client proteins that demonstrated a change in their protein expression after Hsp90 inhibition, signifying an alteration in the canonical pathways of cell cycle progression, apoptosis, cell invasion, angiogenesis, and metastasis.
Members of the glycolysis pathway were upregulated, demonstrating increased dependency on glycolysis for energy source by the treated glioma cells.
Upregulated proteins also include Hsp70 and members of its family such as Hsp27 and gp96, thereby suggesting the role of Hsp90 co-chaperones in compensating for Hsp90 function after Hsp90 inhibition.
Considering Hsp70’s role in antiapoptosis, it was postulated that a combination therapy involving a multitarget approach could be carried out.
Consequently inhibition of both Hsp90 and Hsp70 in U87-MG glioma cells resulted in 60% cell death indicating the importance of combination therapy for glioma therapeutics.
American Psychological Association (APA)
Munje, Chinmay& Shervington, Leroy& Khan, Zarine& Shervington, Amal. 2014. Could Upregulated Hsp70 Protein Compensate for the Hsp90-Silence-Induced Cell Death in Glioma Cells?. International Journal of Brain Science،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-488453
Modern Language Association (MLA)
Munje, Chinmay…[et al.]. Could Upregulated Hsp70 Protein Compensate for the Hsp90-Silence-Induced Cell Death in Glioma Cells?. International Journal of Brain Science No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-488453
American Medical Association (AMA)
Munje, Chinmay& Shervington, Leroy& Khan, Zarine& Shervington, Amal. Could Upregulated Hsp70 Protein Compensate for the Hsp90-Silence-Induced Cell Death in Glioma Cells?. International Journal of Brain Science. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-488453
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488453