Therapeutic Angiogenesis Using Basic Fibroblast Growth Factor in Combination with a Collagen Matrix in Chronic Hindlimb Ischemia
Joint Authors
Yin, Zhenyu
Liu, Pingguo
Liu, Yun
Zhang, Sheng
Zhao, Yilin
Wang, Jinling
Zhou, Jianyin
Wang, Xiaomin
Liu, Zhengjin
Zhen, Maochuan
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Although therapeutic angiogenesis by angiogenic cytokines is a feasible strategy to improve regional blood flow in ischemic regions, the optimal delivery mode needs to be established.
Here we designed a complex of collagen matrix (CM) and basic fibroblast growth factor (bFGF) and evaluated its proangiogenic effect in ischemic hindlimbs.
The bFGF-CM was prepared using lyophilization.
The morphology, porosity and toxicity of CM were examined.
The bFGF releasing profile and bioactivity of released bFGF were assessed.
bFGF-CM was intramuscularly implanted into the rabbit ischemic hindlimb model.
Oxygen saturation parameters (OSP) of ischemic hindlimbs was measured to evaluate the extremity perfusion at intervals.
Histological examination was performed to evaluate the level of angiogenesis.
The CM and bFGF-CM were of identical multiporous structure lacking cytotoxicity.
The releasing profile lasted 10 days and the released bFGF remained bioactive.
OSP in bFGF-CM group was significantly higher than that in CM, bFGF and ischemic groups at 2 and 4 weeks.
The number of capillaries and mature vessels in bFGF-CM group were significantly greater than that in untreated control, CM and bFGF groups.
Therefore, bFGF-CM enables the safe and effective long-term release of bFGF with improved angiogenesis in ischemic hindlimbs compared with CM devoid of bFGF.
American Psychological Association (APA)
Zhou, Jianyin& Zhao, Yilin& Wang, Jinling& Zhang, Sheng& Liu, Zhengjin& Zhen, Maochuan…[et al.]. 2012. Therapeutic Angiogenesis Using Basic Fibroblast Growth Factor in Combination with a Collagen Matrix in Chronic Hindlimb Ischemia. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-488472
Modern Language Association (MLA)
Zhou, Jianyin…[et al.]. Therapeutic Angiogenesis Using Basic Fibroblast Growth Factor in Combination with a Collagen Matrix in Chronic Hindlimb Ischemia. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-488472
American Medical Association (AMA)
Zhou, Jianyin& Zhao, Yilin& Wang, Jinling& Zhang, Sheng& Liu, Zhengjin& Zhen, Maochuan…[et al.]. Therapeutic Angiogenesis Using Basic Fibroblast Growth Factor in Combination with a Collagen Matrix in Chronic Hindlimb Ischemia. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-488472
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488472