Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene
Joint Authors
Gao, Mingyang
Ma, Dexing
Li, Guangxing
Huang, Xiaodan
Niu, Ze
Ma, Chunli
Source
Journal of Parasitology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We previously reported that the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15, fused through linking Eimeria acervulina 3-1E encoding gene and mature chicken IL-15 (mChIL-15) gene with four flexible amino acid SPGS, could significantly offer protection against homologous challenge.
In the present study, the induction of cellular immune response induced by the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15 was investigated.
Spleen lymphocyte subpopulations were characterized by flow cytometric analysis.
The spleen lymphocyte proliferation assays were measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide (MTT) method.
The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR.
Chickens immunized with pcDNA-3-1E-linker-mChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen following two immunizations compared with chickens in other groups (P<0.01).
In comparison with pcDNA3.1-immunized and control groups, lymphocyte proliferation, percentage of CD8α+ cell, and levels of ChIL-2 and ChIFN-γ transcripts in the group immunized with pcDNA-3-1E-linker-mChIL-15 were significantly increased on day 6 following challenge (P<0.05, P<0.01, and P<0.01, resp.).
Our data suggested that the fusion antigen 3-1E-linker-mChIL-15 could be a potential candidate for E.
acervulina vaccine development.
American Psychological Association (APA)
Ma, Dexing& Ma, Chunli& Gao, Mingyang& Li, Guangxing& Niu, Ze& Huang, Xiaodan. 2012. Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene. Journal of Parasitology Research،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-488611
Modern Language Association (MLA)
Ma, Dexing…[et al.]. Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene. Journal of Parasitology Research No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-488611
American Medical Association (AMA)
Ma, Dexing& Ma, Chunli& Gao, Mingyang& Li, Guangxing& Niu, Ze& Huang, Xiaodan. Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene. Journal of Parasitology Research. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-488611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488611