Analyzing Gene Expression Profile in K562 Cells Exposed to Sodium Valproate Using Microarray Combined with the Connectivity Map Database

Joint Authors

Lin, Dong-Jun
Wang, Chun-Huai
Ding, Qian
Zhang, Xiang-Zhong
Zhu, Xiao-Yu
Yin, Ai-Hua
Chen, Yun-Xian

Source

Journal of Biomedicine and Biotechnology

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-06-04

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

To explore the mechanism underlying antileukaemia effect of sodium valproate, the growth and survival of the K562 cell line were investigated.

Global profiles of gene expression in K562 cells exposed to sodium valproate were assessed and validated.

The differentially expressed genes identified were further used to query the connectivity map database to retrieve a ranked list of compounds that act on the same intracellular targets as sodium valproate.

A significant increase in cell apoptosis and a change in gene expression profile were observed in valproate-exposed K562 cells.

The significant enrichment analysis of gene ontology terms for the differentially expressed genes showed that these genes were involved in many important biological processes.

Eight differentially expressed genes involved in apoptosis were verified by quantitative real-time PCR.

The connectivity map analysis showed gene expression profile in K562 cells exposed to sodium valproate was most similar to that of HDACi and PI3K inhibitors, suggesting that sodium valproate might exert antileukaemic action by inhibiting HDAC as well as inhibiting PI3K pathway.

In conclusion, our data might provide clues to elucidate the molecular and therapeutic potential of VPA in leukaemia treatment, and the connectivity map is a useful tool for exploring the molecular mechanism of drug action.

American Psychological Association (APA)

Zhang, Xiang-Zhong& Yin, Ai-Hua& Lin, Dong-Jun& Zhu, Xiao-Yu& Ding, Qian& Wang, Chun-Huai…[et al.]. 2012. Analyzing Gene Expression Profile in K562 Cells Exposed to Sodium Valproate Using Microarray Combined with the Connectivity Map Database. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-488615

Modern Language Association (MLA)

Zhang, Xiang-Zhong…[et al.]. Analyzing Gene Expression Profile in K562 Cells Exposed to Sodium Valproate Using Microarray Combined with the Connectivity Map Database. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-488615

American Medical Association (AMA)

Zhang, Xiang-Zhong& Yin, Ai-Hua& Lin, Dong-Jun& Zhu, Xiao-Yu& Ding, Qian& Wang, Chun-Huai…[et al.]. Analyzing Gene Expression Profile in K562 Cells Exposed to Sodium Valproate Using Microarray Combined with the Connectivity Map Database. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-488615

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-488615