In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication
Joint Authors
Mehrbod, Parvaneh
Tejo, Bimo Ario
Omar, Abdul Rahman
Choong, Oi Kuan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Feline Infectious Peritonitis (FIP) is a severe fatal immune-augmented disease in cat population.
It is caused by FIP virus (FIPV), a virulent mutant strain of Feline Enteric Coronavirus (FECV).
Current treatments and prophylactics are not effective.
The in vitro antiviral properties of five circular Triple-Helix Forming Oligonucleotide (TFO) RNAs (TFO1 to TFO5), which target the different regions of virulent feline coronavirus (FCoV) strain FIPV WSU 79-1146 genome, were tested in FIPV-infected Crandell-Rees Feline Kidney (CRFK) cells.
RT-qPCR results showed that the circular TFO RNAs, except TFO2, inhibit FIPV replication, where the viral genome copy numbers decreased significantly by 5-fold log10 from 1014 in the virus-inoculated cells to 109 in the circular TFO RNAs-transfected cells.
Furthermore, the binding of the circular TFO RNA with the targeted viral genome segment was also confirmed using electrophoretic mobility shift assay.
The strength of binding kinetics between the TFO RNAs and their target regions was demonstrated by NanoITC assay.
In conclusion, the circular TFOs have the potential to be further developed as antiviral agents against FIPV infection.
American Psychological Association (APA)
Choong, Oi Kuan& Mehrbod, Parvaneh& Tejo, Bimo Ario& Omar, Abdul Rahman. 2014. In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-488649
Modern Language Association (MLA)
Choong, Oi Kuan…[et al.]. In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-488649
American Medical Association (AMA)
Choong, Oi Kuan& Mehrbod, Parvaneh& Tejo, Bimo Ario& Omar, Abdul Rahman. In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-488649
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488649