A Simple and Efficient Method to Isolate LTR Sequences of Plant Retrotransposon
Joint Authors
Hou, Xiao-Gai
Guo, Da-Long
Zhang, Xi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Retrotransposons (RTNs) have important roles in the formation of plant genome size, structure, and evolution.
Ubiquitous distributions, abundant copy numbers, high heterogeneities, and insertional polymorphisms of RTNs have made them as excellent sources for molecular markers development.
However, the wide application of RTNs-based molecular markers is restricted by the scarcity of the LTR (long terminal repeat) sequences information.
A new, simple, and efficient method to isolate LTR sequences of RTNs was presented based on the degenerate RNase H nested primers and PPT (polypurine tract) primer of RTNs in tree peony.
This method combined the characteristics and advantages of high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR), annealing control primer (ACP) system, and suppression PCR method.
Nineteen LTR sequences were isolated using this new method in tree peony and the applicability of the LTR sequences based markers was validated by further SSAP analysis.
The results showed that the new method is simple, of low-cost, and highly efficient, which is just conducted by three rounds of PCR and does not need any restriction enzymes and adapters, much less the hybridizations.
This new method is rapid, economical, and cost- and time-saving, which could be easily used to isolate LTR sequences of RTNs.
American Psychological Association (APA)
Guo, Da-Long& Hou, Xiao-Gai& Zhang, Xi. 2014. A Simple and Efficient Method to Isolate LTR Sequences of Plant Retrotransposon. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-488899
Modern Language Association (MLA)
Guo, Da-Long…[et al.]. A Simple and Efficient Method to Isolate LTR Sequences of Plant Retrotransposon. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-488899
American Medical Association (AMA)
Guo, Da-Long& Hou, Xiao-Gai& Zhang, Xi. A Simple and Efficient Method to Isolate LTR Sequences of Plant Retrotransposon. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-488899
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488899