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Method Development and Validation of Darifenacin in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry : Application to a Pharmacokinetic Study
Joint Authors
Karavadi, Thejomoorthy
Challa, B. R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A selective, sensitive, and high-throughput liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) method has been developed and validated for the quantitation of darifenacin in rat plasma.
Sample clean up involved liquid-liquid extraction (LLE) and used 100 μL of rat plasma.
Zorbax, SB C18, 4.6×75 mm, 3.5 μm particle size analytical column using 10 mM ammonium acetate buffer (pH 5) and methanol (10 : 90, v/v) as the mobile phase was used.
The parent → product ion transitions for the drug (m/z 427.3 → 147.3) and IS (m/z 431.4 → 151.2) were monitored on a triple quadrupole mass spectrometer, operating in the multiple reaction monitoring (MRM) and positive ion mode.
The method was validated over the concentration range of 10.00–20000.00 pg/mL for darifenacin.
The method was successfully applied into a pharmacokinetic study in rat plasma under fasting conditions.
American Psychological Association (APA)
Karavadi, Thejomoorthy& Challa, B. R.. 2012. Method Development and Validation of Darifenacin in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry : Application to a Pharmacokinetic Study. ISRN Spectroscopy،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-488985
Modern Language Association (MLA)
Karavadi, Thejomoorthy& Challa, B. R.. Method Development and Validation of Darifenacin in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry : Application to a Pharmacokinetic Study. ISRN Spectroscopy No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-488985
American Medical Association (AMA)
Karavadi, Thejomoorthy& Challa, B. R.. Method Development and Validation of Darifenacin in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry : Application to a Pharmacokinetic Study. ISRN Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-488985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488985