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Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns
Joint Authors
Siddiqi, M. S.
Kumar, Ratan
Upadhyay, Nitin K.
Gupta, Asheesh
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The present investigation was undertaken to evaluate the healing efficacy of lyophilized aqueous leaf extract of Sea buckthorn (Hippophae rhamnoides L., family Elaeagnaceae) (SBT) and to explore its possible mechanism of action on experimental burn wounds in rats.
The SBT extract, at various concentrations, was applied topically, twice daily for 7 days.
Treatment with silver sulfadiazine (SSD) ointment was used as reference control.
The most effective concentration of the extract was found to be 5.0% (w/w) for burn wound healing and this was further used for detailed study.
The SBT-treated group showed faster reduction in wound area in comparison with control and SSD-treated groups.
The topical application of SBT increased collagen synthesis and stabilization at the wound site, as evidenced by increase in hydroxyproline, hexosamine levels and up-regulated expression of collagen type-III.
The histological examinations and matrix metalloproteinases (MMP-2 and -9) expression also confirmed the healing efficacy of SBT leaf extract.
Furthermore, there was significant increase in levels of endogenous enzymatic and non-enzymatic antioxidants and decrease in lipid peroxide levels in SBT-treated burn wound granulation tissue.
The SBT also promoted angiogenesis as evidenced by an in vitro chick chorioallantoic membrane model and in vivo up-regulated vascular endothelial growth factor (VEGF) expression.
The SBT leaf extract had no cytotoxic effect on BHK-21 cell line.
In conclusion, SBT aqueous leaf extract possesses significant healing potential in burn wounds and has a positive influence on the different phases of wound repair.
American Psychological Association (APA)
Upadhyay, Nitin K.& Kumar, Ratan& Siddiqi, M. S.& Gupta, Asheesh. 2011. Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-489048
Modern Language Association (MLA)
Upadhyay, Nitin K.…[et al.]. Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-489048
American Medical Association (AMA)
Upadhyay, Nitin K.& Kumar, Ratan& Siddiqi, M. S.& Gupta, Asheesh. Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-489048
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489048