Enhanced Expression of NR2B Subunits of NMDA Receptors in the Inherited Glaucomatous DBA2J Mouse Retina
Joint Authors
Miao, Yanying
Dong, Ling-Dan
Wu, Jihong
Chen, Jie
Wang, Zhongfeng
Li, Fang
Gao, Feng
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
DBA/2J mouse has been used as a model for spontaneous secondary glaucoma.
Here, we investigated changes in expression of NMDA receptor (NMDAR) subunits and Cdk5/p35/NMDAR signaling in retinas of DBA/2J mice using Western blot technique.
The protein levels of NR1 and NR2A subunits in retinas of DBA/2J mice at all ages (6–12 months) were not different from those in age-matched C57BL/6 mice.
In contrast, the protein levels of NR2B subunits, in addition to age-dependent change, significantly increased with elevated intraocular pressure (IOP) in DBA/2J mice at 6 and 9 months as compared with age-matched controls.
Moreover, expression of Cdk5, p35 and ratio of p-NR2AS1232/NR2A progressively increased with time in both strains, suggestive of activated Cdk5/p35 signaling pathway.
However, the changes in these proteins were in the same levels in both strain mice, except a significant increase of p35 proteins at 6 months in DBA/2J mice.
Meanwhile, the protein levels of Brn-3a, a retinal ganglion cell (RGC) maker, remarkably decreased at 9–12 months in DBA/2J mice, which was in parallel with the changes of NR2B expression.
Our results suggest that elevated IOP-induced increase in expression of NR2B subunits of NMDARs may be involved in RGC degeneration of DBA/2J mice.
American Psychological Association (APA)
Dong, Ling-Dan& Chen, Jie& Li, Fang& Gao, Feng& Wu, Jihong& Miao, Yanying…[et al.]. 2013. Enhanced Expression of NR2B Subunits of NMDA Receptors in the Inherited Glaucomatous DBA2J Mouse Retina. Neural Plasticity،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-489086
Modern Language Association (MLA)
Dong, Ling-Dan…[et al.]. Enhanced Expression of NR2B Subunits of NMDA Receptors in the Inherited Glaucomatous DBA2J Mouse Retina. Neural Plasticity No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-489086
American Medical Association (AMA)
Dong, Ling-Dan& Chen, Jie& Li, Fang& Gao, Feng& Wu, Jihong& Miao, Yanying…[et al.]. Enhanced Expression of NR2B Subunits of NMDA Receptors in the Inherited Glaucomatous DBA2J Mouse Retina. Neural Plasticity. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-489086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489086