Immunogenicity Studies of Bivalent Inactivated Virions of EV71CVA16 Formulated with Submicron Emulsion Systems
Joint Authors
Chong, Pele
Liu, Shih-Jen
Chow, Yen-Hung
Lu, Tsung-Chun
Huang, Ming-Hsi
Liu, Chia-Chyi
Lin, Chih-Wei
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We assessed two strategies for preparing candidate vaccines against hand, foot, and mouth disease (HFMD) caused mainly by infections of enterovirus (EV) 71 and coxsackievirus (CV) A16.
We firstly design and optimize the potency of adjuvant combinations of emulsion-based delivery systems, using EV71 candidate vaccine as a model.
We then perform immunogenicity studies in mice of EV71/CVA16 antigen combinations formulated with PELC/CpG.
A single dose of inactivated EV71 virion (0.2 μg) emulsified in submicron particles was found (i) to induce potent antigen-specific neutralizing antibody responses and (ii) consistently to elicit broad antibody responses against EV71 neutralization epitopes.
A single dose immunogenicity study of bivalent activated EV71/CVA16 virion formulated with either Alum or PELC/CpG adjuvant showed that CVA16 antigen failed to elicit CVA16 neutralizing antibody responses and did not affect EV71-specific neutralizing antibody responses.
A boosting dose of emulsified EV71/CVA16 bivalent vaccine candidate was found to be necessary to achieve high seroconversion of CVA16-specific neutralizing antibody responses.
The current results are important for the design and development of prophylactic vaccines against HFMD and other emerging infectious diseases.
American Psychological Association (APA)
Lin, Chih-Wei& Liu, Chia-Chyi& Lu, Tsung-Chun& Liu, Shih-Jen& Chow, Yen-Hung& Chong, Pele…[et al.]. 2014. Immunogenicity Studies of Bivalent Inactivated Virions of EV71CVA16 Formulated with Submicron Emulsion Systems. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-489120
Modern Language Association (MLA)
Lin, Chih-Wei…[et al.]. Immunogenicity Studies of Bivalent Inactivated Virions of EV71CVA16 Formulated with Submicron Emulsion Systems. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-489120
American Medical Association (AMA)
Lin, Chih-Wei& Liu, Chia-Chyi& Lu, Tsung-Chun& Liu, Shih-Jen& Chow, Yen-Hung& Chong, Pele…[et al.]. Immunogenicity Studies of Bivalent Inactivated Virions of EV71CVA16 Formulated with Submicron Emulsion Systems. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-489120
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489120