Purification and Characterization of Phenylalanine Ammonia Lyase from Trichosporon cutaneum
Joint Authors
Scaman, Christine H.
Goldson-Barnaby, Andrea
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Trichosporon cutaneum phenylalanine ammonia lyase was selected as a model to investigate the dual substrate activity of this family of enzymes.
Sequencing of the PAL gene identified an extensive intron region at the N-terminus.
Five amino acid residues differing from a prior report were identified.
Highest Phe : Tyr activities (1.6 ±0.3 : 0.4±0.1 μmol/h g wet weight) were induced by Tyr.
The enzyme has a temperature optimum of 32°C and a pH optimum of 8–8.5 and shows no metal cofactor dependence.
Michaelis-Menten kinetics (Phe, Km 5.0 ± 1.1 mM) and positive allostery (Tyr, K′ 2.4 ± 0.6 mM, Hill coefficient 1.9±0.5) were observed.
Anion exchange chromatography gave a purification fold of 50 with 20% yield.
The His-Gln motif (substrate selectivity switch region) indicates the enzyme’s ability to act on both substrates.
American Psychological Association (APA)
Goldson-Barnaby, Andrea& Scaman, Christine H.. 2013. Purification and Characterization of Phenylalanine Ammonia Lyase from Trichosporon cutaneum. Enzyme Research،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-489144
Modern Language Association (MLA)
Goldson-Barnaby, Andrea& Scaman, Christine H.. Purification and Characterization of Phenylalanine Ammonia Lyase from Trichosporon cutaneum. Enzyme Research No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-489144
American Medical Association (AMA)
Goldson-Barnaby, Andrea& Scaman, Christine H.. Purification and Characterization of Phenylalanine Ammonia Lyase from Trichosporon cutaneum. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-489144
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489144