Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH
Joint Authors
Viana Silva, José Roberto
Landim-Alvarenga, Fernanda Da Cruz
Toniolli, Ricardo
Seneda, Marcelo Marcondes
Maddox-Hyttel, Poul
Andrade, Evelyn Rabelo
Alfieri, Amauri Alcindo
Figueiredo, José Ricardo
Source
Veterinary Medicine International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH.
To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy.
Except in the control, primordial follicles cultured in supplemented media for 6 d were ultrastructurally normal.
They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed.
Rough endoplasmic reticulum (RER) was rarely found.
The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER.
In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.
American Psychological Association (APA)
Andrade, Evelyn Rabelo& Maddox-Hyttel, Poul& Landim-Alvarenga, Fernanda Da Cruz& Viana Silva, José Roberto& Alfieri, Amauri Alcindo& Seneda, Marcelo Marcondes…[et al.]. 2010. Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH. Veterinary Medicine International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-489161
Modern Language Association (MLA)
Andrade, Evelyn Rabelo…[et al.]. Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH. Veterinary Medicine International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-489161
American Medical Association (AMA)
Andrade, Evelyn Rabelo& Maddox-Hyttel, Poul& Landim-Alvarenga, Fernanda Da Cruz& Viana Silva, José Roberto& Alfieri, Amauri Alcindo& Seneda, Marcelo Marcondes…[et al.]. Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH. Veterinary Medicine International. 2010. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-489161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489161