![](/images/graphics-bg.png)
Microtubules Growth Rate Alteration in Human Endothelial Cells
Joint Authors
Verin, Alexander D.
Alieva, Irina B.
Black, Stephen M.
Wiseman, Dean A.
Gorshkov, Boris A.
Kireev, Igor I.
Zemskov, Evgeny A.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-04-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
To understand how microtubules contribute to the dynamic reorganization of the endothelial cell (EC) cytoskeleton, we established an EC model expressing EB3-GFP, a protein that marks microtubule plus-ends.
Using this model, we were able to measure microtubule growth rate at the centrosome region and near the cell periphery of a single human EC and in the EC monolayer.
We demonstrate that the majority of microtubules in EC are dynamic, the growth rate of their plus-ends is highest in the internal cytoplasm, in the region of the centrosome.
Growth rate of microtubule plus-ends decreases from the cell center toward the periphery.
Our data suggest the existing mechanism(s) of local regulation of microtubule plus-ends growth in EC.
Microtubule growth rate in the internal cytoplasm of EC in the monolayer is lower than that of single EC suggesting the regulatory effect of cell-cell contacts.
Centrosomal microtubule growth rate distribution in single EC indicated the presence of two subpopulations of microtubules with “normal” (similar to those in monolayer EC) and “fast” (three times as much) growth rates.
Our results indicate functional interactions between cell-cell contacts and microtubules.
American Psychological Association (APA)
Alieva, Irina B.& Zemskov, Evgeny A.& Kireev, Igor I.& Gorshkov, Boris A.& Wiseman, Dean A.& Black, Stephen M.…[et al.]. 2010. Microtubules Growth Rate Alteration in Human Endothelial Cells. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-489206
Modern Language Association (MLA)
Alieva, Irina B.…[et al.]. Microtubules Growth Rate Alteration in Human Endothelial Cells. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-489206
American Medical Association (AMA)
Alieva, Irina B.& Zemskov, Evgeny A.& Kireev, Igor I.& Gorshkov, Boris A.& Wiseman, Dean A.& Black, Stephen M.…[et al.]. Microtubules Growth Rate Alteration in Human Endothelial Cells. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-489206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489206