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Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations
Joint Authors
Revanasiddappa, Hosakere Doddarevanna
Deepakumari, Hemavathi Nagaraju
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Three simple, sensitive, accurate, and rapid visible spectrophotometric methods (A, B, and C) have been developed for the estimation of nitrazepam in both pure and in pharmaceutical preparations.
They are based on the diazotization of reduced nitrazepam with nitrous acid followed by coupling with acetyl acetone (method A), diphenylamine (method B), and citrazinic acid (method C) to form colored azo-dyes, exhibiting absorption maxima (λmax) at 400, 550, and 460 nm, for methods A, B, and C, respectively.
The produced colored azo-dyes are stable for more than 2 h.
Beer's law was obeyed in the concentration range of 0.5–20, 0.3–14 and 0.5–12 μg/mL for methods A, B, and C, respectively and the corresponding molar absorptivity values are 1.01×104, 1.00×104, and 1.51×104 L mol−1cm−1.
All variables have been optimized and the results were statistically compared with those of a literature method by employing the Student's t-test and F-test.
No interference was observed from common adjuvants normally added to the tablets.
The results obtained in the proposed methods are in good agreement with labeled amounts, when marketed pharmaceutical preparations are analyzed, which could be applied in the routine quality control analysis laboratory.
American Psychological Association (APA)
Deepakumari, Hemavathi Nagaraju& Revanasiddappa, Hosakere Doddarevanna. 2012. Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations. Journal of Spectroscopy،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-489222
Modern Language Association (MLA)
Deepakumari, Hemavathi Nagaraju& Revanasiddappa, Hosakere Doddarevanna. Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations. Journal of Spectroscopy No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-489222
American Medical Association (AMA)
Deepakumari, Hemavathi Nagaraju& Revanasiddappa, Hosakere Doddarevanna. Spectrophotometric Estimation of Nitrazepam in Pure and in Pharmaceutical Preparations. Journal of Spectroscopy. 2012. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-489222
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489222