A Novel Biosensor to Detect MicroRNAs Rapidly

Abstract EN

δ-free F0F1-ATPase within chromatophore was constructed as a novel biosensor to detect miRNA targets.

Specific miRNA probes were linked to each rotary β subunits of F0F1-ATPase.

Detection of miRNAs was based on the proton flux change induced by light-driven rotation of δ-free F0F1-ATPase.

The hybridization reaction was indicated by changes in the fluorescent intensity of pH-sensitive CdTe quantum dots.

Our results showed that the assay was attomole sensitivities (1.2×10−18 mol) to target miRNAs and capable of distinguishing among miRNA family members.

Moreover, the method could be used to monitor real-time hybridization without any complicated fabrication before hybridization.

Thus, the rotary biosensor is not only sensitive and specific to detect miRNA target but also easy to perform.

The δ-free F0F1-ATPase-based rotary biosensor may be a promising tool for the basic research and clinical application of miRNAs.