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Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells
Joint Authors
Yuan, Jiao
Chen, Beibei
Skogerbø, Geir
Chen, Runsheng
Luo, Huaxia
Zhang, Bo
Source
International Journal of Cell Biology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Introduction.
Small noncoding RNAs have important regulatory functions in different cell pathways.
It is believed that most of them mainly play role in gene post-transcriptional regulation in the cytoplasm.
Recent evidence suggests miRNA and siRNA activity in the nucleus.
Here, we show distinct genome-wide sub-cellular localization distribution profiles of small noncoding RNAs in human breast cancer cells.
Methods.
We separated breast cancer cell nuclei from cytoplasm, and identified small RNA sequences using a high-throughput sequencing platform.
To determine the relationship between miRNA sub-cellular distribution and cancer progression, we used microarray analysis to examine the miRNA expression levels in nucleus and cytoplasm of three human cell lines, one normal breast cell line and two breast cancer cell lines.
Logistic regression and SVM were used for further analysis.
Results.
The sub-cellular distribution of small noncoding RNAs shows that numerous miRNAs and their isoforms (isomiR) not only locate to the cytoplasm but also appeare in the nucleus.
Subsequent microarray analyses indicated that the miRNA nuclear-cytoplasmic-ratio is a significant characteristic of different cancer cell lines.
Conclusions.
Our results indicate that the sub-cellular distribution is important for miRNA function, and that the characterization of the small RNAs sub-cellular localizome may contribute to cancer research and diagnosis.
American Psychological Association (APA)
Chen, Beibei& Zhang, Bo& Luo, Huaxia& Yuan, Jiao& Skogerbø, Geir& Chen, Runsheng. 2012. Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells. International Journal of Cell Biology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-489291
Modern Language Association (MLA)
Chen, Beibei…[et al.]. Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells. International Journal of Cell Biology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-489291
American Medical Association (AMA)
Chen, Beibei& Zhang, Bo& Luo, Huaxia& Yuan, Jiao& Skogerbø, Geir& Chen, Runsheng. Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells. International Journal of Cell Biology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-489291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489291