Impaired Caveolae Function and Upregulation of Alternative Endocytic Pathways Induced by Experimental Modulation of Intersectin-1s Expression in Mouse Lung Endothelium
Joint Authors
Wang, Minhua
Bardita, Cristina
Predescu, Dan N.
Predescu, Sanda A.
Neamu, Radu
Source
Biochemistry Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-26
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Intersectin-1s (ITSN-1s), a protein containing five SH3 (A-E) domains, regulates via the SH3A the function of dynamin-2 (dyn2) at the endocytic site.
ITSN-1s expression was modulated in mouse lung endothelium by liposome delivery of either a plasmid cDNA encoding myc-SH3A or a specific siRNA targeting ITSN-1 gene.
The lung vasculature of SH3A-transduced and ITSN-1s- deficient mice was perfused with gold albumin (Au-BSA) to analyze by electron microscopy the morphological intermediates and pathways involved in transendothelial transport or with dinitrophenylated (DNP)-BSA to quantify by ELISA its transport.
Acute modulation of ITSN-1s expression decreased the number of caveolae, impaired their transport, and opened the interendothelial junctions, while upregulating compensatory nonconventional endocytic/transcytotic structures.
Chronic inhibition of ITSN-1s further increased the occurrence of nonconventional intermediates and partially restored the junctional integrity.
These findings indicate that ITSN-1s expression is required for caveolae function and efficient transendothelial transport.
Moreover, our results demonstrate that ECs are highly adapted to perform their transport function while maintaining lung homeostasis.
American Psychological Association (APA)
Predescu, Dan N.& Neamu, Radu& Bardita, Cristina& Wang, Minhua& Predescu, Sanda A.. 2012. Impaired Caveolae Function and Upregulation of Alternative Endocytic Pathways Induced by Experimental Modulation of Intersectin-1s Expression in Mouse Lung Endothelium. Biochemistry Research International،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-489318
Modern Language Association (MLA)
Predescu, Dan N.…[et al.]. Impaired Caveolae Function and Upregulation of Alternative Endocytic Pathways Induced by Experimental Modulation of Intersectin-1s Expression in Mouse Lung Endothelium. Biochemistry Research International No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-489318
American Medical Association (AMA)
Predescu, Dan N.& Neamu, Radu& Bardita, Cristina& Wang, Minhua& Predescu, Sanda A.. Impaired Caveolae Function and Upregulation of Alternative Endocytic Pathways Induced by Experimental Modulation of Intersectin-1s Expression in Mouse Lung Endothelium. Biochemistry Research International. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-489318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489318