A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study
Joint Authors
Jadeja, Yashwantsinh
Patel, Madhavi
Shah, Anamik
Dabhi, Batuk
Jebaliya, Hetal
Source
Chromatography Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-04
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form.
A SunFire C18 (250 × 4.5 mm, 5 μm particle size) column has been used for HPLC and BEH C18 (100 × 2.1 mm, 1.7 μm particle size) column used for UPLC.
The Mobile phase consisted of Methanol : Water (70 : 30) for HPLC and Methanol : Water (55 : 45 v/v) for UPLC.
Isocratic flow was set at 1 mL/min and 0.30 mL/min, respectively, for HPLC and UPLC.
For both HPLC and UPLC system detection has been performed at 211 nm with 30°C column oven temperature (good elution was obtained at 30°C) and injection volume, respectively, 2 μL and 20 μL for HPLC and UPLC.
American Psychological Association (APA)
Jebaliya, Hetal& Patel, Madhavi& Jadeja, Yashwantsinh& Dabhi, Batuk& Shah, Anamik. 2013. A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study. Chromatography Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-489368
Modern Language Association (MLA)
Jebaliya, Hetal…[et al.]. A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study. Chromatography Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-489368
American Medical Association (AMA)
Jebaliya, Hetal& Patel, Madhavi& Jadeja, Yashwantsinh& Dabhi, Batuk& Shah, Anamik. A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-489368
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489368