Telomere Attrition Occurs during Ex Vivo Expansion of Human Dental Pulp Stem Cells
Joint Authors
Vokurkova, Doris
Ivancakova, Romana
Soukup, Tomas
Bouchal, Jan
Karbanova, Jana
Visek, Benjamin
Brcakova, Eva
Suchanek, Jakub
Micuda, Stanislav
Mokry, Jaroslav
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We provide a detailed characteristic of stem cells isolated and expanded from the human dental pulp.
Dental pulp stem cells express mesenchymal cell markers STRO-1, vimentin, CD29, CD44, CD73, CD90, CD166, and stem cell markers Sox2, nestin, and nucleostemin.
They are multipotent as shown by their osteogenic and chondrogenic potential.
We measured relative telomere length in 11 dental pulp stem cell lines at different passages by quantitative real-time PCR.
Despite their large proliferative capacity, stable viability, phenotype, and genotype over prolonged cultivation, human dental pulp stem cells suffer from progressive telomere shortening over time they replicate in vitro.
Relative telomere length (T/S) was inversely correlated with cumulative doubling time.
Our findings indicate that excessive ex vivo expansion of adult stem cells should be reduced at minimum to avoid detrimental effects on telomere maintenance and measurement of telomere length should become a standard when certificating the status and replicative age of stem cells prior therapeutic applications.
American Psychological Association (APA)
Mokry, Jaroslav& Soukup, Tomas& Micuda, Stanislav& Karbanova, Jana& Visek, Benjamin& Brcakova, Eva…[et al.]. 2010. Telomere Attrition Occurs during Ex Vivo Expansion of Human Dental Pulp Stem Cells. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-489398
Modern Language Association (MLA)
Mokry, Jaroslav…[et al.]. Telomere Attrition Occurs during Ex Vivo Expansion of Human Dental Pulp Stem Cells. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-489398
American Medical Association (AMA)
Mokry, Jaroslav& Soukup, Tomas& Micuda, Stanislav& Karbanova, Jana& Visek, Benjamin& Brcakova, Eva…[et al.]. Telomere Attrition Occurs during Ex Vivo Expansion of Human Dental Pulp Stem Cells. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-489398
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489398