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Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NODSCIDIL2Rγnull mice
Joint Authors
Tirelli, Valentina
Ferro, Leda
Riviere, Isabelle
Kachala, Stefan
Sadelain, Michel
Whitsett, Carolyn
Migliaccio, Anna Rita
Masiello, Francesca
Ghinassi, Barbara
Sanchez, Massimo
Migliaccio, Giovanni
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Ex vivo expanded erythroblasts (EBs) may serve as advanced transfusion products provided that lodgment occurs in the macrophage-niche of the marrow permitting maturation.
EBs expanded from adult and cord blood expressed the receptors (CXCR4, VLA-4, and P-selectin ligand 1) necessary for interaction with macrophages.
However, 4-days following transfusion to intact NOD/SCID/IL2Rγnull mice, CD235apos EBs were observed inside CD235aneg splenic cells suggesting that they underwent phagocytosis.
When splenectomized and intact NOD/SCID/IL2Rγnull mice were transfused using retrovirally labeled human EBs, human cells were visualized by bioluminescence imaging only in splenectomized animals.
Four days after injection, human CD235apos cells were detected in marrow and liver of splenectomized mice but only in spleen of controls.
Human CD235apos erythrocytes in blood remained low in all cases.
These studies establish splenectomized NOD/SCID/IL2Rγnull mice as a suitable model for tracking and quantification of human EBs in vivo.
American Psychological Association (APA)
Ghinassi, Barbara& Ferro, Leda& Masiello, Francesca& Tirelli, Valentina& Sanchez, Massimo& Migliaccio, Giovanni…[et al.]. 2011. Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NODSCIDIL2Rγnull mice. Stem Cells International،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-489428
Modern Language Association (MLA)
Ghinassi, Barbara…[et al.]. Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NODSCIDIL2Rγnull mice. Stem Cells International No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-489428
American Medical Association (AMA)
Ghinassi, Barbara& Ferro, Leda& Masiello, Francesca& Tirelli, Valentina& Sanchez, Massimo& Migliaccio, Giovanni…[et al.]. Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NODSCIDIL2Rγnull mice. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-489428
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489428