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Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells
Joint Authors
Rao Chamallamudi, Mallikarjuna
Kutty Nampurath, Gopalan
John, Jessy
Reddy, Neetinkumar D.
Nampoothiri, Madhavan
Kumar, Nitesh
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Insulin is a cytokine which promotes cell growth.
Recently, a few published reports on insulin in different cell lines support the antiapoptotic effect of insulin.
But the reports fail to explain the role of insulin in modulating glutamate-mediated neuronal cell death through excitotoxicity.
Thus, we examined the neuroprotective effect of insulin on glutamate-induced toxicity on differentiated SH-SY5Y neuronal cells.
Changes in cell viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) based assay, while apoptotic damage was detected by acridine orange/ethidium bromide and Hoechst staining.
Intracellular reactive oxygen species (ROS) accumulation and morphological alterations were also measured.
Treatment with glutamate induced apoptosis, elevated ROS levels and caused damage to neurons.
Insulin was able to attenuate the glutamate-induced excitotoxic damage to neuronal cells.
American Psychological Association (APA)
Nampoothiri, Madhavan& Reddy, Neetinkumar D.& John, Jessy& Kumar, Nitesh& Kutty Nampurath, Gopalan& Rao Chamallamudi, Mallikarjuna. 2014. Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells. Behavioural Neurology،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-489467
Modern Language Association (MLA)
Nampoothiri, Madhavan…[et al.]. Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells. Behavioural Neurology No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-489467
American Medical Association (AMA)
Nampoothiri, Madhavan& Reddy, Neetinkumar D.& John, Jessy& Kumar, Nitesh& Kutty Nampurath, Gopalan& Rao Chamallamudi, Mallikarjuna. Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells. Behavioural Neurology. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-489467
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489467