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Quantitative Proteomics via High Resolution MS Quantification : Capabilities and Limitations
Joint Authors
Han, Bomie
Knierman, Michael D.
Butler, Jon P.
Higgs, Richard E.
Source
International Journal of Proteomics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Recent improvements in the mass accuracy and resolution of mass spectrometers have led to renewed interest in label-free quantification using data from the primary mass spectrum (MS1) acquired from data-dependent proteomics experiments.
The capacity for higher specificity quantification of peptides from samples enriched for proteins of biological interest offers distinct advantages for hypothesis generating experiments relative to immunoassay detection methods or prespecified peptide ions measured by multiple reaction monitoring (MRM) approaches.
Here we describe an evaluation of different methods to post-process peptide level quantification information to support protein level inference.
We characterize the methods by examining their ability to recover a known dilution of a standard protein in background matrices of varying complexity.
Additionally, the MS1 quantification results are compared to a standard, targeted, MRM approach on the same samples under equivalent instrument conditions.
We show the existence of multiple peptides with MS1 quantification sensitivity similar to the best MRM peptides for each of the background matrices studied.
Based on these results we provide recommendations on preferred approaches to leveraging quantitative measurements of multiple peptides to improve protein level inference.
American Psychological Association (APA)
Higgs, Richard E.& Butler, Jon P.& Han, Bomie& Knierman, Michael D.. 2013. Quantitative Proteomics via High Resolution MS Quantification : Capabilities and Limitations. International Journal of Proteomics،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-489489
Modern Language Association (MLA)
Higgs, Richard E.…[et al.]. Quantitative Proteomics via High Resolution MS Quantification : Capabilities and Limitations. International Journal of Proteomics No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-489489
American Medical Association (AMA)
Higgs, Richard E.& Butler, Jon P.& Han, Bomie& Knierman, Michael D.. Quantitative Proteomics via High Resolution MS Quantification : Capabilities and Limitations. International Journal of Proteomics. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-489489
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489489