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Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis : Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein
Joint Authors
Yen, Kuo-Chen
Chen, Ming-Wei
Jan, Tong-Rong
Wey, Shiaw-Pyng
Chiang, Chao-Chih
Wun, Tze-Chein
Liao, Mei-Hsiu
Chi, Chin-Wen
Yen, Tzu-Chen
Liao, Tse-Zung
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Radiolabeled annexin V (ANV) has been widely used for imaging cell apoptosis.
Recently, a novel ANV-Kunitz-type protease inhibitor fusion protein, ANV-6L15, was found to be a promising probe for improved apoptosis detection based on its higher affinity to phosphatidylserine (PS) compared to native ANV.
The present paper investigates the feasibility of apoptosis detection using radioiodinated ANV-6L15.
Native ANV and ANV-6L15 were labeled with iodine-123 and iodine-125 using Iodogen method.
The binding between the radioiodinated proteins and erythrocyte ghosts or chemical-induced apoptotic cells was examined.
ANV-6L15 can be radioiodinated with high yield (40%−60%) and excellent radiochemical purity (>95%).
123I-ANV-6L15 exhibited a higher binding ratio to erythrocyte ghosts and apoptotic cells compared to 123I-ANV.
The biodistribution of 123I-ANV-6L15 in mice was also characterized.
123I-ANV-6L15 was rapidly cleared from the blood.
High uptake in the liver and the kidneys may limit the evaluation of apoptosis in abdominal regions.
Our data suggest that radiolabled ANV-6L15 may be a better scintigraphic tracer than native ANV for apoptosis detection.
American Psychological Association (APA)
Liao, Mei-Hsiu& Jan, Tong-Rong& Chiang, Chao-Chih& Yen, Kuo-Chen& Liao, Tse-Zung& Chen, Ming-Wei…[et al.]. 2011. Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis : Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-489608
Modern Language Association (MLA)
Liao, Mei-Hsiu…[et al.]. Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis : Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-489608
American Medical Association (AMA)
Liao, Mei-Hsiu& Jan, Tong-Rong& Chiang, Chao-Chih& Yen, Kuo-Chen& Liao, Tse-Zung& Chen, Ming-Wei…[et al.]. Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis : Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-489608
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489608