Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80
Joint Authors
Lee, Richard E.
Takayama, Kuni
Wang, Cindy
Vestling, Martha M.
Mahrous, Engy A.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The addition of polyoxyethylene sorbitan monooleate (Tween 80) to a culture of mycobacteria greatly influences cell permeability and sensitivity to antibiotics but very little is known regarding the underlying mechanism.
Here we show that Corynebacterium matruchotii (surrogate of mycobacteria) converts Tween 80 to a structural series of polyoxyethylenic acids which are then used to form novel series-2A and series-2B glycolipids.
Minor series-3 glycolipids were also synthesized.
The polyoxyethylenic acids replaced corynomycolic acids in the cell wall.
Correspondingly the trehalose dicorynomycolate content was reduced.
MALDI mass spectrometry, MS-MS, 1H-NMR, and 13C-NMR were used to characterize the series-2 glycolipids.
Series-2A glycolipid is trehalose 6-C36:2-corynomycolate-6′-polyoxyethylenate and series-2B glycolipid is trehalose 6-C36:2-corynomycolate-6′-furan ring-containing polyoxyethylenate.
Mycobacterium smegmatis grown in the presence of Tween 80 also synthesizes series-2 type glycolipids.
The synthesis of these novel glycolipids in corynebacteria and mycobacteria should result in gross changes in the cell wall permeability and drug sensitivity.
American Psychological Association (APA)
Wang, Cindy& Mahrous, Engy A.& Lee, Richard E.& Vestling, Martha M.& Takayama, Kuni. 2010. Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80. Journal of Lipids،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-489686
Modern Language Association (MLA)
Wang, Cindy…[et al.]. Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80. Journal of Lipids No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-489686
American Medical Association (AMA)
Wang, Cindy& Mahrous, Engy A.& Lee, Richard E.& Vestling, Martha M.& Takayama, Kuni. Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80. Journal of Lipids. 2010. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-489686
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489686