Nitroglycerine-Induced Nitrate Tolerance Compromises Propofol Protection of the Endothelial Cells against TNF-α : The Role of PKC-β2 and NADPH Oxidase
Joint Authors
Zhang, Liangqing
Qiao, Xin
Yang, Qing-jun
Liu, Huimin
Du, Yun
Lei, Shao-Qing
Su, Wating
Xia, Zhong-yuan
Xia, Zhengyuan
Xu, Jin-Jin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Continuous treatment with organic nitrates causes nitrate tolerance and endothelial dysfunction, which is involved with protein kinase C (PKC) signal pathway and NADPH oxidase activation.
We determined whether chronic administration with nitroglycerine compromises the protective effects of propofol against tumor necrosis factor (TNF-) induced toxicity in endothelial cells by PKC-β2 dependent NADPH oxidase activation.
Primary cultured human umbilical vein endothelial cells were either treated or untreated with TNF-α (40 ng/mL) alone or in the presence of the specific PKC-β2 inhibitor CGP53353 (1 μM)), nitroglycerine (10 μM), propofol (100 μM), propofol plus nitroglycerin, or CGP53353 plus nitroglycerine, respectively, for 24 hours.
TNF-α increased the levels of superoxide, Nox (nitrate and nitrite), malondialdehyde, and nitrotyrosine production, accompanied by increased protein expression of p-PKC-β2, gP91phox, and endothelial cell apoptosis, whereas all these changes were further enhanced by nitroglycerine.
CGP53353 and propofol, respectively, reduced TNF-α induced oxidative stress and cell toxicity.
CGP53353 completely prevented TNF-α induced oxidative stress and cell toxicity in the presence or absence of nitroglycerine, while the protective effects of propofol were neutralized by nitroglycerine.
It is concluded that nitroglycerine comprises the protective effects of propofol against TNF-α stimulation in endothelial cells, primarily through PKC-β2 dependent NADPH oxidase activation.
American Psychological Association (APA)
Lei, Shao-Qing& Su, Wating& Liu, Huimin& Xu, Jin-Jin& Xia, Zhong-yuan& Yang, Qing-jun…[et al.]. 2013. Nitroglycerine-Induced Nitrate Tolerance Compromises Propofol Protection of the Endothelial Cells against TNF-α : The Role of PKC-β2 and NADPH Oxidase. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-489772
Modern Language Association (MLA)
Lei, Shao-Qing…[et al.]. Nitroglycerine-Induced Nitrate Tolerance Compromises Propofol Protection of the Endothelial Cells against TNF-α : The Role of PKC-β2 and NADPH Oxidase. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-489772
American Medical Association (AMA)
Lei, Shao-Qing& Su, Wating& Liu, Huimin& Xu, Jin-Jin& Xia, Zhong-yuan& Yang, Qing-jun…[et al.]. Nitroglycerine-Induced Nitrate Tolerance Compromises Propofol Protection of the Endothelial Cells against TNF-α : The Role of PKC-β2 and NADPH Oxidase. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-489772
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489772