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Coordinate Transcriptomic and Metabolomic Effects of the Insulin Sensitizer Rosiglitazone on Fundamental Metabolic Pathways in Liver, Soleus Muscle, and Adipose Tissue in Diabetic dbdb Mice
Joint Authors
Brun, Manuel
de Montrion, Catherine
Galizzi, Jean-Pierre
Guigal-Stephan, Nolwen
Le Bouter, Sabrina
Croixmarie, Vincent
Dacquet, Catherine
Boursier-Neyret, Claire
Xuereb, Laura
Lonchampt, Michel
Rodriguez, Marianne
Lockhart, Brian-Paul
Courtade-Gaïani, Sophie
Ktorza, Alain
Umbdenstock, Thierry
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-10
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Rosiglitazone (RSG), developed for the treatment of type 2 diabetes mellitus, is known to have potent effects on carbohydrate and lipid metabolism leading to the improvement of insulin sensitivity in target tissues.
To further assess the capacity of RSG to normalize gene expression in insulin-sensitive tissues, we compared groups of 18-day-treated db/db mice with increasing oral doses of RSG (10, 30, and 100 mg/kg/d) with untreated non-diabetic littermates (db/+).
For this aim, transcriptional changes were measured in liver, inguinal adipose tissue (IAT) and soleus muscle using microarrays and real-time PCR.
In parallel, targeted metabolomic assessment of lipids (triglycerides (TGs) and free fatty acids (FFAs)) in plasma and tissues was performed by UPLC-MS methods.
Multivariate analyses revealed a relationship between the differential gene expressions in liver and liver trioleate content and between blood glucose levels and a combination of differentially expressed genes measured in liver, IAT, and muscle.
In summary, we have integrated gene expression and targeted metabolomic data to present a comprehensive overview of RSG-induced changes in a diabetes mouse model and improved the molecular understanding of how RSG ameliorates diabetes through its effect on the major insulin-sensitive tissues.
American Psychological Association (APA)
Le Bouter, Sabrina& Rodriguez, Marianne& Guigal-Stephan, Nolwen& Courtade-Gaïani, Sophie& Xuereb, Laura& de Montrion, Catherine…[et al.]. 2010. Coordinate Transcriptomic and Metabolomic Effects of the Insulin Sensitizer Rosiglitazone on Fundamental Metabolic Pathways in Liver, Soleus Muscle, and Adipose Tissue in Diabetic dbdb Mice. PPAR Research،Vol. 2010, no. 2010, pp.1-17.
https://search.emarefa.net/detail/BIM-489843
Modern Language Association (MLA)
Le Bouter, Sabrina…[et al.]. Coordinate Transcriptomic and Metabolomic Effects of the Insulin Sensitizer Rosiglitazone on Fundamental Metabolic Pathways in Liver, Soleus Muscle, and Adipose Tissue in Diabetic dbdb Mice. PPAR Research No. 2010 (2010), pp.1-17.
https://search.emarefa.net/detail/BIM-489843
American Medical Association (AMA)
Le Bouter, Sabrina& Rodriguez, Marianne& Guigal-Stephan, Nolwen& Courtade-Gaïani, Sophie& Xuereb, Laura& de Montrion, Catherine…[et al.]. Coordinate Transcriptomic and Metabolomic Effects of the Insulin Sensitizer Rosiglitazone on Fundamental Metabolic Pathways in Liver, Soleus Muscle, and Adipose Tissue in Diabetic dbdb Mice. PPAR Research. 2010. Vol. 2010, no. 2010, pp.1-17.
https://search.emarefa.net/detail/BIM-489843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489843