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Dysfunction of Collagen Synthesis and Secretion in Chondrocytes Induced by Wisp3 Mutation
Joint Authors
Wu, Xian-Ping
Luo, Xiang-Hang
Liu, Ya-Qing
Sheng, Zhi-Feng
Wang, Min
Liao, Er-Yuan
Guo, Li-Juan
Zhou, Hou-De
Man, Xiao-Fei
Source
International Journal of Endocrinology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Wisp3 gene mutation was shown to cause spondyloepiphyseal dysplasia tarda with progressive arthropathy (SRDT-PA), but the underlying mechanism is not clear.
To clarify this mechanism, we constructed the wild and mutated Wisp3 expression vectors and transfected into human chondrocytes lines C-20/A4; Wisp3 proteins subcellular localization, cell proliferation, cell apoptosis, and Wisp3-mediated gene expression were determined, and dynamic secretion of collagen in transfected chondrocytes was analyzed by 14C-proline incorporation experiment.
Mutated Wisp3 protein increased proliferation activity, decreased apoptosis of C-20/A4 cells, and aggregated abnormally in cytoplasm.
Expression of collagen II was also downregulated in C-20/A4 cells transfected with mutated Wisp3.
Wild type Wisp3 transfection increased intracellular collagen content and extracellular collagen secretion, but the mutated Wisp3 lost this function, and the peak phase of collagen secretion was delayed in mutated Wisp3 transfected cells.
Thus abnormal protein distribution, cell proliferation, collagen synthesis, and secretion in Wisp3 mutated chondrocytes might contribute to the pathogenesis of SEDT-PA.
American Psychological Association (APA)
Wang, Min& Man, Xiao-Fei& Liu, Ya-Qing& Liao, Er-Yuan& Sheng, Zhi-Feng& Luo, Xiang-Hang…[et al.]. 2013. Dysfunction of Collagen Synthesis and Secretion in Chondrocytes Induced by Wisp3 Mutation. International Journal of Endocrinology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-489913
Modern Language Association (MLA)
Wang, Min…[et al.]. Dysfunction of Collagen Synthesis and Secretion in Chondrocytes Induced by Wisp3 Mutation. International Journal of Endocrinology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-489913
American Medical Association (AMA)
Wang, Min& Man, Xiao-Fei& Liu, Ya-Qing& Liao, Er-Yuan& Sheng, Zhi-Feng& Luo, Xiang-Hang…[et al.]. Dysfunction of Collagen Synthesis and Secretion in Chondrocytes Induced by Wisp3 Mutation. International Journal of Endocrinology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-489913
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489913