Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom
Joint Authors
Zuliani, Juliana P.
Setúbal, Sulamita da S.
Martins Soares, Andreimar
Kayano, Anderson M.
Furtado, Juliana L.
Stábeli, Rodrigo G.
Lima, Beatriz F.
Lacouth-Silva, Fabianne
Calderon, Leonardo A.
Xavier, Caroline V.
Zaqueo, Kayena D.
Oliveira, George A.
Pontes, Adriana S.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In the present study, we investigated the in vitro effects of two basic myotoxic phospholipases A2 (PLA2), BaTX-I, a catalytically inactive Lys-49 variant, and BaTX-II, a catalytically active Asp-49, and of one acidic myotoxic PLA2, BaPLA2, a catalytically active Asp-49, isolated from Bothrops atrox snake venom, on the activation of J774A.1 macrophages.
At noncytotoxic concentrations, the toxins did not affect the adhesion of the macrophages, nor their ability to detach.
The data obtained showed that only BaTX-I stimulated complement receptor-mediated phagocytosis.
However, BaTX-I, BaTX-II, and BaPLA2 induced the release of the superoxide anion by J774A.1 macrophages.
Additionally, only BaTX-I raised the lysosomal volume of macrophages after 15 min of incubation.
After 30 min, all the phospholipases increased this parameter, which was not observed within 60 min.
Moreover, BaTX-I, BaTX-II, and BaPLA2 increased the number of lipid bodies on macrophages submitted to phagocytosis and not submitted to phagocytosis.
However, BaTX-II and BaPLA2 induced the release of TNF-α by J774A.1 macrophages.
Taken together, the data show that, despite differences in enzymatic activity, the three toxins induced inflammatory events and whether the enzyme is acidic or basic does not seem to contribute to these effects.
American Psychological Association (APA)
Furtado, Juliana L.& Oliveira, George A.& Pontes, Adriana S.& Setúbal, Sulamita da S.& Xavier, Caroline V.& Lacouth-Silva, Fabianne…[et al.]. 2014. Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-490226
Modern Language Association (MLA)
Furtado, Juliana L.…[et al.]. Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-490226
American Medical Association (AMA)
Furtado, Juliana L.& Oliveira, George A.& Pontes, Adriana S.& Setúbal, Sulamita da S.& Xavier, Caroline V.& Lacouth-Silva, Fabianne…[et al.]. Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-490226
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490226