Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation
Joint Authors
Kamble, Rajashri D.
Jadhav, Anandrao R.
Source
International Journal of Microbiology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
A thermoalkalophilic new species of Bacillus, similar to Bacillus arseniciselenatis DSM 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source.
The extracellular xylanase was isolated by ammonium sulfate (80%) precipitation and purified using ion exchange chromatography.
The molecular weight of xylanase was ~29.8 kDa.
The optimum temperature and pH for the enzyme activity were 50°C and pH 8.0.
The enzyme was active on birchwood xylan and little active on p-nitrophenyl xylopyranoside but not on Avicel, CMC, cellobiose, and starch, showing its absolute substrate specificity.
For birchwood xylan, the enzyme gave a Km 5.26 mg/mL and Vmax 277.7 μmol/min/mg, respectively.
In addition, the xylanase was also capable of producing high-quality xylo-oligosaccharides, which indicated its application potential not only in pulp biobleaching processes but also in the nutraceutical industry.
American Psychological Association (APA)
Kamble, Rajashri D.& Jadhav, Anandrao R.. 2012. Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation. International Journal of Microbiology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-490234
Modern Language Association (MLA)
Kamble, Rajashri D.& Jadhav, Anandrao R.. Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation. International Journal of Microbiology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-490234
American Medical Association (AMA)
Kamble, Rajashri D.& Jadhav, Anandrao R.. Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation. International Journal of Microbiology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-490234
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490234