RhoROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change
Joint Authors
Ma, Li
Guo, Han-bin
Cao, Jian-biao
Zhang, Yan
Zhou, Yi-ming
Lan, Xi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Asymmetric dimethylarginine (ADMA) induces vascular smooth muscle cells (VSMCs) migration.
VSMC phenotype change is a prerequisite of migration.
RhoA and Rho-kinase (ROCK) mediate migration of VSMCs.
We hypothesize that ADMA induces VSMC migration via the activation of Rho/ROCK signal pathway and due to VSMCs phenotype change.
ADMA activates Rho/ROCK signal pathway that interpreted by the elevation of RhoA activity and phosphorylation level of a ROCK substrate.
Pretreatment with ROCK inhibitor, Y27632 completely reverses the induction of ADMA on ROCK and in turn inhibits ADMA-induced VSMCs migration.
When the Rho/ROCK signal pathway has been blocked by pretreatment with Y27632, the induction of ERK signal pathway by ADMA is completely abrogated.
Elimination of ADMA via overexpression of dimethylarginine dimethylaminohydrolase 2 (DDAH2) and L-arginine both blocks the effects of ADMA on the activation of Rho/ROCK and extra cellular signal-regulated kinase (ERK) in VSMCs.
The expression of differentiated phenotype relative proteins was reduced and the actin cytoskeleton was disassembled by ADMA, which were blocked by Y27632, further interpreting that ADMA inducing VSMCs migration via Rho/ROCK signal pathway is due to its effect on the VSMCs phenotype change.
Our present study may help to provide novel insights into the therapy and prevention of atherosclerosis.
American Psychological Association (APA)
Zhou, Yi-ming& Lan, Xi& Guo, Han-bin& Zhang, Yan& Ma, Li& Cao, Jian-biao. 2014. RhoROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-490275
Modern Language Association (MLA)
Zhou, Yi-ming…[et al.]. RhoROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-490275
American Medical Association (AMA)
Zhou, Yi-ming& Lan, Xi& Guo, Han-bin& Zhang, Yan& Ma, Li& Cao, Jian-biao. RhoROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-490275
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490275