Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells
Joint Authors
Lin, Qiaowei
Li, Lulu
Bao, Zhengxi
Huang, Feiruo
Zheng, Peipei
Wang, Yue
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The present research was undertaken to determine the effects of EPA (20 : 5 n-3) and DHA (22 : 6 n-3) on chylomicron and VLDL synthesis and secretion by Caco-2 cells.
Cells were incubated for 12 to 36 h with 400 μM OA, EPA, and DHA; then 36 h was chosen for further study because EPA and DHA decreased de novo triglycerides synthesis in a longer incubation compared with OA (P<0.01).
Neither the uptake nor oxidation was different in response to the respective fatty acids (P>0.05).
Compared with OA, intercellular and secreted nascent apolipoprotein B48 and B100 were decreased by EPA and DHA (P<0.01).
Both DHA and EPA resulted in a lower secretion of chylomicron and VLDL (P<0.01).
In contrast to OA, EPA and DHA were preferentially incorporated into phospholipids instead of triacylglycerols (P<0.01).
These discoveries demonstrated that exposure of DHA and EPA reduced the secretion of chylomicron and VLDL partly by regulating the synthesis of TG and apoB.
American Psychological Association (APA)
Wang, Yue& Lin, Qiaowei& Zheng, Peipei& Li, Lulu& Bao, Zhengxi& Huang, Feiruo. 2014. Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-490344
Modern Language Association (MLA)
Wang, Yue…[et al.]. Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-490344
American Medical Association (AMA)
Wang, Yue& Lin, Qiaowei& Zheng, Peipei& Li, Lulu& Bao, Zhengxi& Huang, Feiruo. Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Chylomicron and VLDL Synthesis and Secretion in Caco-2 Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-490344
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490344