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Identification of Binding Mode of a Platinum (II) Complex, PtCl2(DIP), and Calf Thymus DNA
Joint Authors
Fatahi, Azadeh
Kashanian, Soheila
Shahabadi, Nahid
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The Pt(II) complex, PtCl2(DIP) (DIP = chelating dinitrogen ligand: 4,7-diphenyl-1,10-phenanthroline), was synthesized and characterized by elemental analysis (CHN) and 1H NMR and UV-vis techniques.
The binding of this complex to calf thymus DNA was investigated using various physicochemical methods such as spectrophotometric, circular dichroism, spectrofluorometric, melting temperature, and viscosimetric techniques.
Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hyperchromism) of calf thymus DNA (CT-DNA): increase in melting temperature, sharp increase in specific viscosity of DNA, and induced CD spectral changes.
Also the fluorescence spectral characteristics and interaction of Pt complex with DNA have been studied.
Pt bound to DNA showed a marked decrease in the fluorescence intensity.
The results show that both the complex and the NR molecules can intercalate competitively into the DNA double-helix structure.
The experimental results show that the mode of binding of the this complex to DNA is classical intercalation.
American Psychological Association (APA)
Shahabadi, Nahid& Kashanian, Soheila& Fatahi, Azadeh. 2011. Identification of Binding Mode of a Platinum (II) Complex, PtCl2(DIP), and Calf Thymus DNA. Bioinorganic Chemistry and Applications،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-490660
Modern Language Association (MLA)
Shahabadi, Nahid…[et al.]. Identification of Binding Mode of a Platinum (II) Complex, PtCl2(DIP), and Calf Thymus DNA. Bioinorganic Chemistry and Applications No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-490660
American Medical Association (AMA)
Shahabadi, Nahid& Kashanian, Soheila& Fatahi, Azadeh. Identification of Binding Mode of a Platinum (II) Complex, PtCl2(DIP), and Calf Thymus DNA. Bioinorganic Chemistry and Applications. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-490660
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490660