MiR-15b Targets Cyclin D1 to Regulate Proliferation and Apoptosis in Glioma Cells
Joint Authors
Wan, Zhengqiang
Li, Min
Yan, Shushan
Sun, Guan
Shi, Lei
Fu, Linshan
Guo, Jun
Jiang, Nan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aim.
To investigate the role and mechanism of miR-15b in the proliferation and apoptosis of glioma.
Methods.
The miR-15b mimics were transfected into human glioma cells to upregulate the miR-15b expression.
Cyclin D1 was determined by both western blotting analysis and luciferase reporter assay.
Methylthiazol tetrazolium (MTT) and flow cytometry were employed to detect the cell proliferation, cell cycle, and apoptosis.
Results.
Overexpression of miR-15b inhibits proliferation by arrested cell cycle progression and induces apoptosis, possibly by directly targeting Cyclin D1.
Both luciferase assay and bioinformatics search revealed a putative target site of miR-15b binding to the 3′-UTR of Cyclin D1.
Moreover, expression of miR-15b in glioma tissues was found to be inversely correlated with Cyclin D1 expression.
Enforced Cyclin D1 could abrogate the miR-15b-mediated cell cycle arrest and apoptosis.
Conclusions.
Our findings identified that miR-15b may function as a glioma suppressor by targeting the Cyclin D1, which may provide a novel therapeutic strategy for treatment of glioma.
American Psychological Association (APA)
Sun, Guan& Shi, Lei& Yan, Shushan& Wan, Zhengqiang& Jiang, Nan& Fu, Linshan…[et al.]. 2014. MiR-15b Targets Cyclin D1 to Regulate Proliferation and Apoptosis in Glioma Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-490672
Modern Language Association (MLA)
Sun, Guan…[et al.]. MiR-15b Targets Cyclin D1 to Regulate Proliferation and Apoptosis in Glioma Cells. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-490672
American Medical Association (AMA)
Sun, Guan& Shi, Lei& Yan, Shushan& Wan, Zhengqiang& Jiang, Nan& Fu, Linshan…[et al.]. MiR-15b Targets Cyclin D1 to Regulate Proliferation and Apoptosis in Glioma Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-490672
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490672