![](/images/graphics-bg.png)
Disruption of Lipid Rafts Interferes with the Interaction of Toxoplasma gondii with Macrophages and Epithelial Cells
Joint Authors
Cruz, Thayana Araújo
Paredes-Santos, Tatiana Christina
de Souza, Wanderley
Veras de Moraes, Gabriela
Attias, Marcia
Cruz, Karla Dias
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The intracellular parasite Toxoplasma gondii can penetrate any warm-blooded animal cell.
Conserved molecular assemblies of host cell plasma membranes should be involved in the parasite-host cell recognition.
Lipid rafts are well-conserved membrane microdomains that contain high concentrations of cholesterol, sphingolipids, glycosylphosphatidylinositol, GPI-anchored proteins, and dually acylated proteins such as members of the Src family of tyrosine kinases.
Disturbing lipid rafts of mouse peritoneal macrophages and epithelial cells of the lineage LLC-MK2 with methyl-beta cyclodextrin (MβCD) and filipin, which interfere with cholesterol or lidocaine, significantly inhibited internalization of T.
gondii in both cell types, although adhesion remained unaffected in macrophages and decreased only in LLC-MK2 cells.
Scanning and transmission electron microscopy confirmed these observations.
Results are discussed in terms of the original role of macrophages as professional phagocytes versus the LLC-MK2 cell lineage originated from kidney epithelial cells.
American Psychological Association (APA)
Cruz, Karla Dias& Cruz, Thayana Araújo& Veras de Moraes, Gabriela& Paredes-Santos, Tatiana Christina& Attias, Marcia& de Souza, Wanderley. 2014. Disruption of Lipid Rafts Interferes with the Interaction of Toxoplasma gondii with Macrophages and Epithelial Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-490676
Modern Language Association (MLA)
Cruz, Karla Dias…[et al.]. Disruption of Lipid Rafts Interferes with the Interaction of Toxoplasma gondii with Macrophages and Epithelial Cells. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-490676
American Medical Association (AMA)
Cruz, Karla Dias& Cruz, Thayana Araújo& Veras de Moraes, Gabriela& Paredes-Santos, Tatiana Christina& Attias, Marcia& de Souza, Wanderley. Disruption of Lipid Rafts Interferes with the Interaction of Toxoplasma gondii with Macrophages and Epithelial Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-490676
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490676