Recombinant Dense Granular Protein (GRA5) for Detection of Human Toxoplasmosis by Western Blot
Joint Authors
Fong, Mun Yik
Nissapatorn, Veeranoot
Ching, Xiao Teng
Lau, Yee Ling
Andiappan, Hemah
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Toxoplasma gondii infects all warm-blooded animals, including humans, causing serious public health problems and great economic loss for the food industry.
Commonly used serological tests require costly and hazardous preparation of whole Toxoplasma lysate antigens from tachyzoites.
Here, we have evaluated an alternative method for antigen production, which involved a prokaryotic expression system.
Specifically, we expressed T.
gondii dense granular protein-5 (GRA5) in Escherichia coli and isolated it by affinity purification.
The serodiagnostic potential of the purified recombinant GRA5 (rGRA5) was tested through Western blot analysis against 212 human patient serum samples.
We found that rGRA5 protein was 100% specific for analysis of toxoplasmosis-negative human sera.
Also, rGRA5 was able to detect acute and chronic T.
gondii infections (sensitivities of 46.8% and 61.2%, resp.).
American Psychological Association (APA)
Ching, Xiao Teng& Lau, Yee Ling& Fong, Mun Yik& Nissapatorn, Veeranoot& Andiappan, Hemah. 2014. Recombinant Dense Granular Protein (GRA5) for Detection of Human Toxoplasmosis by Western Blot. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-490830
Modern Language Association (MLA)
Ching, Xiao Teng…[et al.]. Recombinant Dense Granular Protein (GRA5) for Detection of Human Toxoplasmosis by Western Blot. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-490830
American Medical Association (AMA)
Ching, Xiao Teng& Lau, Yee Ling& Fong, Mun Yik& Nissapatorn, Veeranoot& Andiappan, Hemah. Recombinant Dense Granular Protein (GRA5) for Detection of Human Toxoplasmosis by Western Blot. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-490830
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490830