Ox-LDL Promotes Migration and Adhesion of Bone Marrow-Derived Mesenchymal Stem Cells via Regulation of MCP-1 Expression
Joint Authors
Lu, Ming
Zhang, Fenxi
Wang, Huaibin
Wang, Congrui
Yuan, Zhiqing
Li, Yonghai
Lin, Juntang
Feng, Huigen
Wang, Xianwei
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Bone marrow-derived mesenchymal stem cells (bmMSCs) are the most important cell source for stem cell transplant therapy.
The migration capacity of MSCs is one of the determinants of the efficiency of MSC-based transplant therapy.
Our recent study has shown that low concentrations of oxidized low-density lipoprotein (ox-LDL) can stimulate proliferation of bmMSCs.
In this study, we investigated the effects of ox-LDL on bmMSC migration and adhesion, as well as the related mechanisms.
Our results show that transmigration rates of bmMSCs and cell-cell adhesion between bmMSCs and monocytes are significantly increased by treatments with ox-LDL in a dose- and time-dependent manner.
Expressions of ICAM-1, PECAM-1, and VCAM-1 as well as the levels of intracellular Ca2+ are also markedly increased by ox-LDL in a dose-dependent manner.
Cytoskeleton analysis shows that ox-LDL treatment benefits to spreading of bmMSCs and organization of F-actin fibers after being plated for 6 hours.
More interestingly, treatments with ox-LDL also markedly increase expressions of LOX-1, MCP-1, and TGF-β; however, LOX-1 antibody and MCP-1 shRNA markedly inhibit ox-LDL-induced migration and adhesion of bmMSCs, which suggests that ox-LDL-induced bmMSC migration and adhesion are dependent on LOX-1 activation and MCP-1 expression.
American Psychological Association (APA)
Zhang, Fenxi& Wang, Congrui& Wang, Huaibin& Lu, Ming& Li, Yonghai& Feng, Huigen…[et al.]. 2013. Ox-LDL Promotes Migration and Adhesion of Bone Marrow-Derived Mesenchymal Stem Cells via Regulation of MCP-1 Expression. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-490877
Modern Language Association (MLA)
Zhang, Fenxi…[et al.]. Ox-LDL Promotes Migration and Adhesion of Bone Marrow-Derived Mesenchymal Stem Cells via Regulation of MCP-1 Expression. Mediators of Inflammation No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-490877
American Medical Association (AMA)
Zhang, Fenxi& Wang, Congrui& Wang, Huaibin& Lu, Ming& Li, Yonghai& Feng, Huigen…[et al.]. Ox-LDL Promotes Migration and Adhesion of Bone Marrow-Derived Mesenchymal Stem Cells via Regulation of MCP-1 Expression. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-490877
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490877