On the Mechanism(s) of Membrane Permeability Transition in Liver Mitochondria of Lamprey, Lampetra fluviatilis L. : Insights from Cadmium
Joint Authors
Emelyanova, Larisa V.
Savina, Margarita V.
Belyaeva, Elena A.
Korotkov, Sergey M.
Brailovskaya, Irina V.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-02
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Previously we have shown that opening of the mitochondrial permeability transition pore in its low conductance state is the case in hepatocytes of the Baltic lamprey (Lampetra fluviatilis L.) during reversible metabolic depression taking place in the period of its prespawning migration when the exogenous feeding is switched off.
The depression is observed in the last year of the lamprey life cycle and is conditioned by reversible mitochondrial dysfunction (mitochondrial uncoupling in winter and coupling in spring).
To further elucidate the mechanism(s) of induction of the mitochondrial permeability transition pore in the lamprey liver, we used Cd2+ and Ca2+ plus Pi as the pore inducers.
We found that Ca2+ plus Pi induced the high-amplitude swelling of the isolated “winter” mitochondria both in isotonic sucrose and ammonium nitrate medium while both low and high Cd2+ did not produce the mitochondrial swelling in these media.
Low Cd2+ enhanced the inhibition of basal respiration rate of the “winter” mitochondria energized by NAD-dependent substrates whereas the same concentrations of the heavy metal evoked its partial stimulation on FAD-dependent substrates.
The above changes produced by Cd2+ or Ca2+ plus Pi in the “winter” mitochondria were only weakly (if so) sensitive to cyclosporine A (a potent pharmacological desensitizer of the nonselective pore) added alone and they were not sensitive to dithiothreitol (a dithiol reducing agent).
Under monitoring of the transmembrane potential of the “spring” lamprey liver mitochondria, we revealed that Cd2+ produced its decrease on both types of the respiratory substrates used that was strongly hampered by cyclosporine A, and the membrane potential was partially restored by dithiothreitol.
The effects of different membrane permeability modulators on the lamprey liver mitochondria function and the seasonal changes in their action are discussed.
American Psychological Association (APA)
Belyaeva, Elena A.& Emelyanova, Larisa V.& Korotkov, Sergey M.& Brailovskaya, Irina V.& Savina, Margarita V.. 2014. On the Mechanism(s) of Membrane Permeability Transition in Liver Mitochondria of Lamprey, Lampetra fluviatilis L. : Insights from Cadmium. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-490947
Modern Language Association (MLA)
Belyaeva, Elena A.…[et al.]. On the Mechanism(s) of Membrane Permeability Transition in Liver Mitochondria of Lamprey, Lampetra fluviatilis L. : Insights from Cadmium. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-490947
American Medical Association (AMA)
Belyaeva, Elena A.& Emelyanova, Larisa V.& Korotkov, Sergey M.& Brailovskaya, Irina V.& Savina, Margarita V.. On the Mechanism(s) of Membrane Permeability Transition in Liver Mitochondria of Lamprey, Lampetra fluviatilis L. : Insights from Cadmium. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-490947
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490947