Identification of a Novel Methylated Gene in Nasopharyngeal Carcinoma : TTC40
Joint Authors
Ayadi, Wajdi
Frikha, Hanèn
Daoud, Jamel
Frikha, Mounir
Trigui, Emna
Mokdad-Gargouri, Raja
Ghorbel, Abdelmonem
Allaya, Nesrine
Gargouri, Ali
Khabir, Abdelmajid
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
To further explore the epigenetic changes in nasopharyngeal carcinoma (NPC), methylation-sensitive arbitrarily primed PCR was performed on NPC biopsies and nontumor nasopharyngeal samples.
We have shown mainly two DNA fragments that appeared to be differentially methylated in NPCs versus nontumors.
The first, defined as hypermethylated, corresponds to a CpG island at the 5′-end of the tetratricopeptide repeat domain 40 (TTC40) gene, whereas the second, defined as hypo-methylated, is located on repetitive sequences at chromosomes 16p11.1 and 13.1.
Thereafter, the epigenetic alteration on the 5′-TTC40 gene was confirmed by methylation-specific PCR, showing a significant aberrant methylation in NPCs, compared to nontumors.
In addition, the bisulfite sequencing analysis has shown a very high density of methylated cytosines in C15, C17, and X666 NPC xenografts.
To assess whether TTC40 gene is silenced by aberrant methylation, we examined the gene expression by reverse transcription-PCR.
Our analysis showed that the mRNA expression was significantly lower in tumors than in nontumors, which is associated with 5′-TTC40 gene hypermethylation.
In conclusion, we found that the 5′-TTC40 gene is frequently methylated and is associated with the loss of mRNA expression in NPCs.
Hypermethylation of 5′-TTC40 gene might play a role in NPC development; nevertheless, other studies are needed.
American Psychological Association (APA)
Ayadi, Wajdi& Allaya, Nesrine& Frikha, Hanèn& Trigui, Emna& Khabir, Abdelmajid& Ghorbel, Abdelmonem…[et al.]. 2014. Identification of a Novel Methylated Gene in Nasopharyngeal Carcinoma : TTC40. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-490951
Modern Language Association (MLA)
Ayadi, Wajdi…[et al.]. Identification of a Novel Methylated Gene in Nasopharyngeal Carcinoma : TTC40. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-490951
American Medical Association (AMA)
Ayadi, Wajdi& Allaya, Nesrine& Frikha, Hanèn& Trigui, Emna& Khabir, Abdelmajid& Ghorbel, Abdelmonem…[et al.]. Identification of a Novel Methylated Gene in Nasopharyngeal Carcinoma : TTC40. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-490951
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490951