The Immunomodulatory Effects of Albumin In Vitro and In Vivo
Joint Authors
Denenberg, Alvin
Zingarelli, Basilia
Lahni, Patrick M.
Wong, Hector R.
Giuliano, John S.
Wheeler, Derek S.
Source
Advances in Pharmacological Sciences
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Albumin appears to have proinflammatory effects in vitro.
We hypothesized that albumin would induce a state of tolerance to subsequent administration of lipopolysaccharide (LPS) in vitro and in vivo.
RAW264.7 and primary peritoneal macrophages were treated with increasing doses of bovine serum albumin (BSA) and harvested for NF-κB luciferase reporter assay or TNF-α ELISA.
In separate experiments, RAW264.7 cells were preconditioned with 1 mg/mL BSA for 18 h prior to LPS (10 μg/mL) treatment and harvested for NF-κB luciferase reporter assay or TNF-α ELISA.
Finally, C57Bl/6 mice were preconditioned with albumin via intraperitoneal administration 18 h prior to a lethal dose of LPS (60 mg/kg body wt).
Blood was collected at 6 h after LPS administration for TNF-α ELISA.
Albumin produced a dose-dependent and TLR-4-dependent increase in NF-κB activation and TNF-α gene expression in vitro.
Albumin preconditioning abrogated the LPS-mediated increase in NF-κB activation and TNF-α gene expression in vitro and in vivo.
The clinical significance of these findings remains to be elucidated.
American Psychological Association (APA)
Wheeler, Derek S.& Giuliano, John S.& Lahni, Patrick M.& Denenberg, Alvin& Wong, Hector R.& Zingarelli, Basilia. 2011. The Immunomodulatory Effects of Albumin In Vitro and In Vivo. Advances in Pharmacological Sciences،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-490964
Modern Language Association (MLA)
Wheeler, Derek S.…[et al.]. The Immunomodulatory Effects of Albumin In Vitro and In Vivo. Advances in Pharmacological Sciences No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-490964
American Medical Association (AMA)
Wheeler, Derek S.& Giuliano, John S.& Lahni, Patrick M.& Denenberg, Alvin& Wong, Hector R.& Zingarelli, Basilia. The Immunomodulatory Effects of Albumin In Vitro and In Vivo. Advances in Pharmacological Sciences. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-490964
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490964