Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation
Joint Authors
Cheng, Ching-Yi
Cheng, Yi-Fang
Liang, Yao-Jen
Huang, Chun-Fang
Lin, Jiun-Tsai
Chen, Han-Min
Chen, Chin-Chen
Kao, Shao-Hsuan
Kuo, Cheng-Yi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Adenosine 5′-monophosphate-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis via modulating metabolism of glucose, lipid, and protein.
In addition to energy modulation, AMPK has been demonstrated to associate with several important cellular events including inflammation.
The results showed that ENERGI-F704 identified from bamboo shoot extract was nontoxic in concentrations up to 80 μM and dose-dependently induced phosphorylation of AMPK (Thr-172) in microglia BV2 cells.
Our findings also showed that the treatment of BV2 with ENERGI-F704 ameliorated the LPS-induced elevation of IL-6 and TNF-α production.
In addition, ENERGI-F704 reduced increased production of nitric oxide (NO) and prostaglandin E2 (PGE2) via downregulating the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2), respectively.
Moreover, ENERGI-F704 decreased activated nuclear translocation and protein level of NF-κB.
Inhibition of AMPK with compound C restored decreased NF-κB translocation by ENERGI-F704.
In conclusion, ENERGI-F704 exerts inhibitory activity on LPS-induced inflammation through manipulating AMPK signaling and exhibits a potential therapeutic agent for neuroinflammatory disease.
American Psychological Association (APA)
Chen, Chin-Chen& Lin, Jiun-Tsai& Cheng, Yi-Fang& Kuo, Cheng-Yi& Huang, Chun-Fang& Kao, Shao-Hsuan…[et al.]. 2014. Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-490978
Modern Language Association (MLA)
Chen, Chin-Chen…[et al.]. Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-490978
American Medical Association (AMA)
Chen, Chin-Chen& Lin, Jiun-Tsai& Cheng, Yi-Fang& Kuo, Cheng-Yi& Huang, Chun-Fang& Kao, Shao-Hsuan…[et al.]. Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-490978
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490978